Click image to see more details
-
-
-
-
-
+2
Product Info Summary
| SKU: | M00676-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00676-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody catalog # M00676-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00676-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HII-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hsp27
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00676-2 is reactive to HSPB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
27 kDa
Calculated molecular weight
22783 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00676-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human 293T whole cell, human K562 whole cell
IHC: human colon cancer tissue, rat stomach tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HSPB1 using anti-HSPB1 antibody (M00676-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 antigen affinity purified monoclonal antibody (Catalog # M00676-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB1 at approximately 27 kDa. The expected band size for HSPB1 is at 23 kDa.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:500 dilution.
Click image to see more details
Figure 2. IHC analysis of HSPB1 using anti-HSPB1 antibody (M00676-2).
HSPB1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HSPB1 Antibody (M00676-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Figure 3. IHC analysis of HSPB1 using anti-HSPB1 antibody (M00676-2).
HSPB1 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HSPB1 Antibody (M00676-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For HSPB1 (Source: Uniprot.org, NCBI)
Gene Name
HSPB1
Full Name
Heat shock protein beta-1
Weight
22783 MW
Superfamily
small heat shock protein (HSP20) family
Alternative Names
Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27;HSPB1;HSP27, HSP28; HSPB1 CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27 heat shock protein family B (small) member 1 heat shock protein beta-1|28 kDa heat shock protein|epididymis secretory protein Li 102|estrogen-regulated 24 kDa protein|heat shock 27 kDa protein|heat shock 27kD protein 1|heat shock 27kDa protein 1|stress-responsive protein 27
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPB1, check out the HSPB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody (M00676-2)
Hello CJ!
No publications found for M00676-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody
Question
Does anti-Hsp27 Rabbit Monoclonal antibody M00676-2 work for Flow Cytometry with pancreas?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
According to the expression profile of pancreas, HSPB1 is highly expressed in pancreas. So, it is likely that anti-Hsp27 Rabbit Monoclonal antibody M00676-2 will work for Flow Cytometry with pancreas.
Boster Scientific Support
Answered: 2019-12-04
Question
My question regarding product M00676-2, anti-Hsp27 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00676-2 anti-Hsp27 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-21
Question
I see that the anti-Hsp27 Rabbit Monoclonal antibody M00676-2 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-Hsp27 Rabbit Monoclonal antibody M00676-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-19
Question
We want to test anti-Hsp27 Rabbit Monoclonal antibody M00676-2 on mouse pancreas for research purposes, then I may be interested in using anti-Hsp27 Rabbit Monoclonal antibody M00676-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
The products we sell, including anti-Hsp27 Rabbit Monoclonal antibody M00676-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-09
Question
Is a blocking peptide available for product anti-Hsp27 Rabbit Monoclonal antibody (M00676-2)?
S. Bhatt
Verified customer
Asked: 2018-01-24
Answer
We do provide the blocking peptide for product anti-Hsp27 Rabbit Monoclonal antibody (M00676-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-24
