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Product Info Summary
| SKU: | A02506 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-HSD17B2 Antibody Picoband®
SKU/Catalog Number
A02506
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSD17B2 Antibody Picoband® catalog # A02506. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSD17B2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02506)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02506 is reactive to HSD17B2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
42.785kDa
Background of HSD17B2
HSD17B2 (17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42 (43%) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02506 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human placenta tissue
FCM: CACO-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HSD17B2 using anti-HSD17B2 antibody (A02506).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B2 antigen affinity purified polyclonal antibody (Catalog # A02506) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B2 at approximately 40KD. The expected band size for HSD17B2 is at 40KD.
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Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-HSD17B2 antibody (A02506).
Overlay histogram showing CACO-2 cells stained with A02506 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B2 Antibody (A02506, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For HSD17B2 (Source: Uniprot.org, NCBI)
Gene Name
HSD17B2
Full Name
Estradiol 17-beta-dehydrogenase 2
Weight
42.785kDa
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
Tumor necrosis factor receptor superfamily member 4; MRC OX40; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; Ox40; Txgp1l HSD17B2 EDH17B2, HSD17, SDR9C2 hydroxysteroid 17-beta dehydrogenase 2 17-beta-hydroxysteroid dehydrogenase type 2|17-beta-HSD 2|20 alpha-hydroxysteroid dehydrogenase|20-alpha-HSD|E2DH|estradiol 17-beta-dehydrogenase 2|microsomal 17-beta-hydroxysteroid dehydrogenase|short chain dehydrogenase/reductase family 9C member 2|testosterone 17-beta-dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSD17B2, check out the HSD17B2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSD17B2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSD17B2 Antibody Picoband® (A02506)
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