Anti-HSD17B2 Antibody Picoband™

Product Name

Anti-HSD17B2 Antibody Picoband™

View all HSD17B2 Antibodies

SKU/Catalog Number

A02506

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HSD17B2 Antibody Picoband™ catalog # A02506. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSD17B2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02506)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02506 is reactive to HSD17B2 in Human

Applications

A02506 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

Background of HSD17B2

HSD17B2 (17-BETA-HYDROXYSTEROID DEHYDROGENASE II), also called 17-BETA-HSD II, is an anzyme which have 387 amino acids with a predicted molecular weight of 42,782 and associate with the membranes of the endoplasmic reticulum. Its Cytogenetic location is 16q23.3. The type 2 enzyme was capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. HSD17B2 mRNA was detected in 18 of 42 (43%) adenomas but not in prolactinomas. In the human endometrium, inactivation of 17-beta-estradiol to estrone is catalyzed by HSD17B2. And HSD17B2 activity distinguishing between disease-free and diseased endometria. HSD17B2 efficiently catalyzes the oxidative metabolism of androgens and estrogens, and it is expressed in a large series of human peripheral tissues. The previous paradigm that HSD17B2 activity in the endometrium is elevated during the secretory phase is confined to diseased endometrium but not to disease-free endometrium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of HSD17B2 using anti-HSD17B2 antibody (A02506).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B2 antigen affinity purified polyclonal antibody (Catalog # A02506) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B2 at approximately 40KD. The expected band size for HSD17B2 is at 40KD.

Click image to see more details

Figure 2. Flow Cytometry analysis of CACO-2 cells using anti-HSD17B2 antibody (A02506).
Overlay histogram showing CACO-2 cells stained with A02506 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B2 Antibody (A02506, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For HSD17B2 (Source: Uniprot.org, NCBI)

Alternative Names

17-beta-hydroxysteroid dehydrogenase type 2; 20 alpha-hydroxysteroid dehydrogenase; E2DH; EC 1.1.1.62,17-beta-HSD 2; EC 1.1.1.63; EDH17B220-alpha-HSD; estradiol 17-beta-dehydrogenase 2; HSD17; hydroxysteroid (17-beta) dehydrogenase 2; Microsomal 17-beta-hydroxysteroid dehydrogenase; SDR9C2; short chain dehydrogenase/reductase family 9C, member 2; Testosterone 17-beta-dehydrogenase HSD17B2 EDH17B2, HSD17, SDR9C2 hydroxysteroid 17-beta dehydrogenase 2 17-beta-hydroxysteroid dehydrogenase type 2|17-beta-HSD 2|20 alpha-hydroxysteroid dehydrogenase|20-alpha-HSD|E2DH|estradiol 17-beta-dehydrogenase 2|microsomal 17-beta-hydroxysteroid dehydrogenase|short chain dehydrogenase/reductase family 9C member 2|testosterone 17-beta-dehydrogenase

For more info on HSD17B2, check out the HSD17B2 Infographic

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In this infographic, you will see the following information for HSD17B2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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