Anti-HR23B RAD23B Antibody

Product Name

Anti-HR23B RAD23B Antibody

View all hHR23b Antibodies

SKU/Catalog Number

A02174

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HR23B RAD23B Antibody (Catalog # A02174). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-HR23B RAD23B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02174)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 155-180 of human HR23B protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02174 is reactive to RAD23B in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

43171 MW

Background of hHR23b

HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02174 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For RAD23B (Source: Uniprot.org, NCBI)

Alternative Names

HR23B, hHR23B, mHR23B, p58, RAD23 (S. cerevisiae) homolog B, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein RAD23B HHR23B, HR23B, P58 RAD23 homolog B, nucleotide excision repair protein UV excision repair protein RAD23 homolog B|RAD23, yeast homolog of, B|XP-C repair complementing complex 58 kDa|XP-C repair complementing protein|XP-C repair-complementing complex 58 kDa protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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