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Product Info Summary
| SKU: | PB10071 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HPRT/HPRT1 Antibody Picoband®
SKU/Catalog Number
PB10071
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HPRT/HPRT1 Antibody Picoband® catalog # PB10071. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HPRT/HPRT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10071)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human HPRT recombinant protein (Position: A2-S110). Human HPRT shares 98.1% and 96.3% amino acid (aa) sequence identity with mouse and rat HPRT, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10071 is reactive to HPRT1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
24579 MW
Background of HPRT
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10071 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human Colo320 whole cell, human HepG2 whole cell, human 293T whole cell, rat brain tissue, rat spleen tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HPRT/HPRT1 using anti-HPRT/HPRT1 antibody (PB10071).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Colo320 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT/HPRT1 antigen affinity purified polyclonal antibody (Catalog # PB10071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HPRT/HPRT1 at approximately 25 kDa. The expected band size for HPRT/HPRT1 is at 25 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HPRT1 (Source: Uniprot.org, NCBI)
Gene Name
HPRT1
Full Name
Hypoxanthine-guanine phosphoribosyltransferase
Weight
24579 MW
Superfamily
purine/pyrimidine phosphoribosyltransferase family
Alternative Names
Hypoxanthine-guanine phosphoribosyltransferase;HGPRT;HGPRTase;2.4.2.8;HPRT1;HPRT; HPRT1 HGPRT, HPRT hypoxanthine phosphoribosyltransferase 1 hypoxanthine-guanine phosphoribosyltransferase|HGPRTase|hypoxanthine-guanine phosphoribosyltransferase 1|testicular tissue protein Li 89
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HPRT1, check out the HPRT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HPRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HPRT/HPRT1 Antibody Picoband® (PB10071)
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Customer Q&As
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5 Customer Q&As for Anti-HPRT/HPRT1 Antibody Picoband®
Question
We are currently using anti-HPRT/HPRT1 antibody PB10071 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
The anti-HPRT/HPRT1 antibody (PB10071) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-01
Question
Does PB10071 anti-HPRT/HPRT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Taylor
Verified customer
Asked: 2019-10-23
Answer
It shows on the product datasheet, PB10071 anti-HPRT/HPRT1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-23
Question
Is a blocking peptide available for product anti-HPRT/HPRT1 antibody (PB10071)?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
We do provide the blocking peptide for product anti-HPRT/HPRT1 antibody (PB10071). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-24
Question
Does anti-HPRT/HPRT1 antibody PB10071 work on goat WB with oocyte?
C. Johnson
Verified customer
Asked: 2015-11-19
Answer
Our lab technicians have not validated anti-HPRT/HPRT1 antibody PB10071 on goat. You can run a BLAST between goat and the immunogen sequence of anti-HPRT/HPRT1 antibody PB10071 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat oocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-19
Question
I was wanting to use your anti-HPRT/HPRT1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
A. Rodriguez
Verified customer
Asked: 2014-07-10
Answer
You can see on the product datasheet, PB10071 anti-HPRT/HPRT1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-10
