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Product Info Summary
| SKU: | A07948 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HOXA4 Antibody Picoband®
SKU/Catalog Number
A07948
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HOXA4 Antibody Picoband® catalog # A07948. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HOXA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07948)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HOXA4, which shares 72.2% and 61.1% amino acid (aa) sequence identity with mouse and rat HOXA4, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07948 is reactive to HOXA4 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
49229 MW
Background of HOXA4
HOXA4(HOMEOBOX A4), also known as HOX1D or HOMOLOG OF, is a protein that in humans is encoded by the HOXA4 gene, which is also part of the A cluster on chromosome 7. HOXA4 encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07948 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human
Positive Control
WB: human SW620 whole cell, human 293T whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HOXA4 using anti-HOXA4 antibody (A07948).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA4 antigen affinity purified polyclonal antibody (Catalog # A07948) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA4 at approximately 36 kDa. The expected band size for HOXA4 is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HOXA4 (Source: Uniprot.org, NCBI)
Gene Name
HOXA4
Full Name
Homeobox protein Hox-A4
Weight
49229 MW
Superfamily
Antp homeobox family
Alternative Names
DAZ-associated protein 1; Deleted in azoospermia-associated protein 1; DAZAP1 HOXA4 HOX1, HOX1D homeobox A4 homeobox protein Hox-A4|Dfd-like protein|Hox-1.4-like protein|homeo box A4|homeobox protein Hox-1.4|homeobox protein Hox-1D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HOXA4, check out the HOXA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HOXA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HOXA4 Antibody Picoband® (A07948)
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