Anti-HOXA4 Antibody Picoband™

Product Name

Anti-HOXA4 Antibody Picoband™

View all HOXA4 Antibodies

SKU/Catalog Number

A07948

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HOXA4 Antibody Picoband™ catalog # A07948. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-HOXA4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07948)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human HOXA4, which shares 72.2% and 61.1% amino acid (aa) sequence identity with mouse and rat HOXA4, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07948 is reactive to HOXA4 in Human

Applications

A07948 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

49229 MW

Background of HOXA4

HOXA4(HOMEOBOX A4), also known as HOX1D or HOMOLOG OF, is a protein that in humans is encoded by the HOXA4 gene, which is also part of the A cluster on chromosome 7. HOXA4 encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HOXA4 using anti-HOXA4 antibody (A07948).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA4 antigen affinity purified polyclonal antibody (Catalog # A07948) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA4 at approximately 36 kDa. The expected band size for HOXA4 is at 36 kDa.

Gene/Protein Information For HOXA4 (Source: Uniprot.org, NCBI)

Alternative Names

homeo box A4; homeobox A4; Homeobox protein Hox-1.4; Homeobox protein Hox-1D; homeobox protein Hox-A4; HOX1; Hox-1.4-like protein; HOX1DDfd-like protein HOXA4 HOX1, HOX1D homeobox A4 homeobox protein Hox-A4|Dfd-like protein|Hox-1.4-like protein|homeo box A4|homeobox protein Hox-1.4|homeobox protein Hox-1D

For more info on HOXA4, check out the HOXA4 Infographic

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In this infographic, you will see the following information for HOXA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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