Homeobox protein Hox-A4 Antibodies

Homeobox protein Hox-A4 (HOXA4)

Sequence-specific transcription factor that's part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities.

The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT- 3'. .

Gene Information

Human
Gene Name:	HOXA4
Uniprot:	Q00056
Entrez:	3201

Family

Belongs to:
Antp homeobox family

Alternative Names

homeo box A4; homeobox A4; Homeobox protein Hox-1.4; Homeobox protein Hox-1D; homeobox protein Hox-A4; HOX1; Hox-1.4-like protein; HOX1DDfd-like protein

Molecular Weight

Mass (kDA):
34.499 kDA

Genomic Context

Human
Location:	7p15.2
Sequence:	7; NC_000007.14 (27128525..27130757, complement)

Tissue Specificity

Embryonic nervous system.

Subcellular Localizations

Nucleus.

Diseases

• Leukemia
• Malignant Neoplasms
• Myeloid Leukemia
• Leukemia, Myelocytic, Acute
• Neoplasms
• Nervousness
• Malignant Neoplasm Of Ovary
• Tissue Adhesions
• Carcinoma
• Inflammation
• Ovarian Neoplasm
• Congenital Abnormality
• Malignant Paraganglionic Neoplasm

• Aortic Aneurysm
• Aortic Aneurysm, Abdominal
• Cytogenetic Abnormality
• Cell Invasion
• Leukemogenesis
• Crest Syndrome
• Lymphoid Leukemia

Pathways

• Methylation
• Dna Methylation
• Cell Migration
• Lung Development
• Cell Differentiation
• Localization
• Response To Retinoic Acid
• Pathogenesis
• Cell Adhesion
• Response To Lipopolysaccharide
• Lung Cell Differentiation
• Catagen
• Nucleolar Fragmentation

• Gene Silencing
• Skin Development
• Inflammatory Response
• Translation
• Cell Cycle
• Cell Motility
• Regionalization

PTMs

• Methylation

• Cleavage

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HOXA4

Best Uses Of The HOXA4 Marker

If you want to get the best results, the HOXA4 Marker might be the right one. You might also be curious about the Anti-ADAM12 and Anti-Methyl-Dimethyl-Diphenyl-Methyl (MDDM) markers. This article will help you make an informed decision about which ones to buy.

HOXA4Marker

Multiple types of cancer including lung cancer have been linked with the Wnt Signaling pathway. In lung cancer, HOXA4 overexpression suppresses the Wnt signaling pathway. Western blot analysis of HOXA4 expression revealed that the protein levels of downstream effectors of the Wnt signaling pathway were downregulated in the lung tumor. The study suggests that the HOXA4 Gene may be a useful diagnostic marker for lung Cancer.

Recent studies have shown that HOXA4 gene expression in lung cancer cell line lines inhibits their migration, possibly via the B1 integrin. Because other members in the HOX gene group are overexpressed, it is not clear what HOXA4 does to lung cancer. Therefore, more research is needed in order to understand the role of the HOXA4 gene in lung cancer.

The expression level of HOXA4 has also been significantly lower in lung tumors compared to normal tissues. This could have clinical implications in lung cancer diagnosis. The study also revealed that HOXA4 was a better predictor than a single gene test. Further research is necessary to examine the role played by HOXA4 as a lung cancer diagnostic. This is the first study to determine if lung cancer was caused by the HOXA4 genes.

HOXA4 inhibits the transcription of YAP/TEAD-dependent genes. This protein also acts as a novel repressor for YAP/TEAD transcripts. It has been suggested HOXA4 could inhibit the activity YAP/TEAD. Its effects on TEAD-dependent transcription pathways are not yet known. But recent research indicates that HOXA4 is important for a variety of cancers, from breast tumors to prostate cancer.

Previous studies have found that HOXA4 and HOXA5 transcription are closely related. Previous studies have not proven that HOXA4 is associated with a poorer survival in LUAD patients. Thus, HOXA family genes may serve as potential biomarkers in this disease. Targeted therapies may be possible if HOXA4/HOXA5 can be found to be functionally implicated in the treatment for cancer.

HOXA4 methylation levels were evaluated in 95 CML patients with IM good response or IM resistance, along with twelve normal controls. The methylation levels were lower in IM CML patients with a good response, while hypermethylation rates were significantly higher in IM CML patients. Consequently, HOXA4 methylation levels could be useful in predicting the outcome CML therapy.

Transfecting cells with the indicated lentiviruses in order to detect HOXA4 genes expression in lung cancer cells led to some results. 48 hours after infection, qRTPCR was used for the detection of HOXA4 gene expression. These experiments revealed that HOXA4 promotes growth of lung cancer cells within mice. The two genes were successfully transfected to the A549- and HCC827 cell line lines for this study.

Anti-ADAM12

In addition to a unique cytoplasmic domain, ADAM12 is required for the progression of the cell cycle. It contains three domains to inhibit this process, namely the cytoplasmic, transmembrane, and disintegrin. These domains can't be replaced by ADAM9 which is a closely related protein. Anti-ADAM12 usage is essential for myoblast differentiation.

ADAM12 siRNAs were used in order to block its expression and observed a significant decrease in CSC population. Anti-ADAM12 antibody increased CD24 transcription, a marker that the non CSC population is present. In the same way, shADAM12 knockdown reduced non-CSC cell number in cells treated with Doxycycline.

ADAM12 plays a role in the induction of myoblast quiescence. Transgenic expression of ADAM12 should be done in myoblasts prior differentiation. After that, it is possible to test their muscle-regeneration capacity in ADAM12 transgenic animal. If this approach succeeds, it will be an effective tool to study ADAM12's role in muscle recovery.

The anti-ADAM12 SiRNA targets the region between nucleotides 3130 & 3148 in the ADAM12 CDNA. Dharmacon Research supplied both the antisense as well as sense siRNAs. As a control, we used the green fluorescent protein duplex to evaluate the nonspecific effects of anti-ADAM12 siRNA. Cells containing 1% FBS were able to detect the anti-ADAM12 SIRNA.

The ADAM12 gene has also been linked with EMT and CSCs. These processes contribute to breast cancer drug resistance. Increased ADAM12 expression has been linked with lower metastasis-free survival. This gene may be a therapeutic target to ER-negative breast tumors. It is also highly expressed within breast tumors with a low claudin phenotype.

The expression of ADAM12 inhibits cell entry into S-phase. Transfected C2C12 cell lines were incubated with 10 mM BrdU and 1% FBS. Cells were stained with anti–ADAM12 and anti–BrdU antibodies twenty-four hours after transfection. This is compared with 80% for cells expressing nascent ADAM12.

ADAM12 supports CSC phenotypes of claudinlow breast cancer cells through modulating the EGFR pathway. In addition, ADAM12 binds beta-1 integrin and inhibits cell spreading in tumor cells. ADAM12 also has a cysteine-switch motif that binds a Zinc ion. This allows for activation. However, therapy resistance has been shown to be associated with ADAM12 expression within cancer cells.

A mouse monoclonal monoclonal antibodies against ADAM12 (clone MABT1331) is being used successfully for flow-cytometry and immunoprecipitation. The antibody can detect human ADAM12 in cell samples, as demonstrated by the results. This antibody is an efficient and convenient method of ADAM 12 detection. If you want to detect the protein, just infuse it with a monoclonal antibody against ADAM12 and run a HOXA4 marker.

Anti-Methyl-Dimethyl-Diphenyl-Dimethyl-Diphenyl-Dimethyl-Diphenyl-Dimethyl-Methyl-Diphenyl-Dimethyl-Methyl-Diphenyl-Methyl-Diphenyl-Dimethyl-Methyl

Common genetic markers for myelodysplastic disease (MDS) include the HOXA4 genes. The gene encodes a transcription factor that regulates the length of telomeres in the human body. MDS is characterized by abnormally shortening of telomeres in the cells. Recent research compared 40 MDS patients and 40 healthy controls to determine the mRNA level for the HOXA4 protein.

The HOXA4 protein gene is expressed in most of the cells of our bodies. It is found at a low expression in the endometrium. However it is abundant in the nucleus, where high levels ERI+ and GPER exist. In endometrial hyperplasia, GPER and ERI+ were highly expressed, with ERI+ being more abundant in cells with columnar architecture. GPER gene levels declined with increasing severity of the condition, from simple and complex to complex. ERI+ density was also higher in atypical than in atypical endometrium.

The HOXA4 gene, which is expressed in the pancreas, is thought to play an important part in the development and maintenance of insulin-producing beta cell in the Langerhans islets. This gene is also known as an interaction with IRX3 & NKX2-2, which restricts the generation motor neurons to the correct neural tube region. It is induced with SHH signals.