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Product Info Summary
| SKU: | PA1602 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Homeobox protein Hox-A3 HOXA3 Antibody Picoband®
SKU/Catalog Number
PA1602
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Homeobox protein Hox-A3 HOXA3 Antibody catalog # PA1602. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein Hox-A3 HOXA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1602)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HOXA3, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1602 is reactive to HOXA3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
46 kDa
Calculated molecular weight
46369 MW
Background of HOXA3
HOXA3 (HOMEOBOX A3) is a protein that in humans is encoded by the HOXA3 gene, which is also part of the A cluster on chromosome 7. HOXA3 encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Its cytogenetic location is 7q15.2. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA3 protein is able to complement Hoxd3 deficiency when expressed in the context of the Hoxd3 allele.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1602 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: SW620 Whole Cell
IHC: Human Pancreatic Cancer Tissue
Validation Images & Assay Conditions
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Anti-HOXA3 antibody, PA1602, Western blotting
All lanes: Anti HOXA3 (PA1602) at 0.5ug/ml
WB: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 46KD
Observed bind size: 46KD
Click image to see more details
Anti-HOXA3 antibody, PA1602, IHC(P)
IHC(P): Human Pancreatic Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For HOXA3 (Source: Uniprot.org, NCBI)
Gene Name
HOXA3
Full Name
Homeobox protein Hox-A3
Weight
46369 MW
Superfamily
Antp homeobox family
Alternative Names
Homeobox protein Hox-A3;Homeobox protein Hox-1E;HOXA3;HOX1E; HOXA3 HOX1, HOX1E homeobox A3 homeobox protein Hox-A3|Hox-1.5-like protein|homeo box 1E|homeo box A3|homeobox protein Hox-1E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HOXA3, check out the HOXA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HOXA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein Hox-A3 HOXA3 Antibody Picoband® (PA1602)
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Customer Q&As
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7 Customer Q&As for Anti-Homeobox protein Hox-A3 HOXA3 Antibody Picoband®
Question
We are currently using anti-HOXA3 antibody PA1602 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The anti-HOXA3 antibody (PA1602) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-12
Question
I am interested in to test anti-HOXA3 antibody PA1602 on human uterus for research purposes, then I may be interested in using anti-HOXA3 antibody PA1602 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The products we sell, including anti-HOXA3 antibody PA1602, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-10
Question
Would PA1602 anti-HOXA3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
You can see on the product datasheet, PA1602 anti-HOXA3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-03
Question
Would anti-HOXA3 antibody PA1602 work on zebrafish WB with female gonad?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
Our lab technicians have not validated anti-HOXA3 antibody PA1602 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-HOXA3 antibody PA1602 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish female gonad in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-11
Question
Do you have a BSA free version of anti-HOXA3 antibody PA1602 available?
N. Moore
Verified customer
Asked: 2019-05-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HOXA3 antibody PA1602 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-HOXA3 antibody PA1602. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-07
Question
I was wanting to use your anti-HOXA3 antibody for WB for human uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human uterus identification?
G. Jones
Verified customer
Asked: 2013-06-20
Answer
As indicated on the product datasheet, PA1602 anti-HOXA3 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-20
