Anti-Homeobox protein Nkx-2.6 NKX2-6 Antibody

Product Name

Anti-Homeobox protein Nkx-2.6 NKX2-6 Antibody

View all Nkx2-6 Antibodies

SKU/Catalog Number

A09381-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Homeobox protein Nkx-2.6 NKX2-6 Antibody (Catalog # A09381-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

NKX2-6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Homeobox protein Nkx-2.6 NKX2-6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09381-1)

Host

Rabbit

Contents

NKX2-6 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NKX2-6 antibody was raised against a 15 amino acid synthetic peptide near the center of human NKX2-6. The immunogen is located within amino acids 170 - 220 of NKX2-6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

NKX2-6 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-6 are known to exist; this antibody will detect both isoforms

Reactive Species

A09381-1 is reactive to NKX2-6 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

32121 MW

Background of Nkx2-6

NKX2-6 (NK2 homeobox 6) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-6 is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are thought to be a cause for some congenital heart abnormalities.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09381-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NKX2-6 antibody can be used for detection of NKX2-6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For NKX2-6 (Source: Uniprot.org, NCBI)

Alternative Names

CSX2, NKX2F, NKX4-2, Homeobox protein NK-2 homolog F Nkx2-6|AV044928, Nkx-2., Nkx-2.6, Nkx2., Nkx2.6, Ti, Tix, tinman|NK2 homeobox 6|homeobox protein Nkx-2.6|Drosophila NK2 transcription factor related, locus 6|NK2 transcription factor related, locus 6|homeobox protein NK-2 homolog F

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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