Anti-hnRPD (Ab-83) HNRNPD Antibody

Product Name

Anti-hnRPD (Ab-83) HNRNPD Antibody

View all AUF1 Antibodies

SKU/Catalog Number

A02895

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-hnRPD (Ab-83) HNRNPD Antibody (Catalog # A02895). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-hnRPD (Ab-83) HNRNPD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02895)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized non-phosphopeptide derived from human hnRPD around the phosphorylation site of serine 83 (N-S-S(p)-P-R).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02895 is reactive to HNRNPD in Human

Reconstitution

Calculated molecular weight

38434 MW

Background of AUF1

Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.

Kajita Y., J. Biol. Chem. 270:22167-22175(1995).
Dempsey L.A., Genomics 49:378-384(1998).
Tolnay M., Biochem. J. 338:417-425(1999).
Grosset C., Cell 103:29-40(2000)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02895 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For HNRNPD (Source: Uniprot.org, NCBI)

Alternative Names

Heterogeneous nuclear ribonucleoprotein D0;hnRNP D0;AU-rich element RNA-binding protein 1;HNRNPD;AUF1, HNRPD; HNRNPD AUF1, AUF1A, HNRPD, P37, hnRNPD0 heterogeneous nuclear ribonucleoprotein D heterogeneous nuclear ribonucleoprotein D0|ARE-binding protein AUFI, type A|AU-rich element RNA binding protein 1, 37kDa|hnRNP D0

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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