Product Info Summary
SKU: | M01270 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-HNF1 beta Rabbit Monoclonal Antibody
View all TCF-2/HNF-1 beta Antibodies
SKU/Catalog Number
M01270
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HNF1 beta Rabbit Monoclonal Antibody catalog # M01270. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HNF1 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01270)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HOH-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HNF1 beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01270 is reactive to HNF1B in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated molecular weight
61324 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01270 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Positive Control
WB: Human kidney
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HNF1 beta expression in Human kidney lysate.
Protein Target Info & Infographic
Gene/Protein Information For HNF1B (Source: Uniprot.org, NCBI)
Gene Name
HNF1B
Full Name
Hepatocyte nuclear factor 1-beta
Weight
61324 MW
Superfamily
HNF1 homeobox family
Alternative Names
Hepatocyte nuclear factor 1-beta;HNF-1-beta;HNF-1B;Homeoprotein LFB3;Transcription factor 2;TCF-2;Variant hepatic nuclear factor 1;vHNF1;HNF1B;TCF2; HNF1B ADTKD3, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, RCAD, T2D, TCF-2, TCF2, VHNF1 HNF1 homeobox B hepatocyte nuclear factor 1-beta|HNF1 beta A|homeoprotein LFB3|transcription factor 2, hepatic
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNF1B, check out the HNF1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HNF1 beta Rabbit Monoclonal Antibody (M01270)
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4 Customer Q&As for Anti-HNF1 beta Rabbit Monoclonal Antibody
Question
Can you help my question with product M01270, anti-HNF1 beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01270 anti-HNF1 beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-15
Question
Is a blocking peptide available for product anti-HNF1 beta Rabbit Monoclonal antibody (M01270)?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
We do provide the blocking peptide for product anti-HNF1 beta Rabbit Monoclonal antibody (M01270). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-02
Question
I am looking for to test anti-HNF1 beta Rabbit Monoclonal antibody M01270 on mouse adult mammalian kidney for research purposes, then I may be interested in using anti-HNF1 beta Rabbit Monoclonal antibody M01270 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
The products we sell, including anti-HNF1 beta Rabbit Monoclonal antibody M01270, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-23
Question
Does anti-HNF1 beta Rabbit Monoclonal antibody M01270 work for WB with adult mammalian kidney?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
According to the expression profile of adult mammalian kidney, HNF1B is highly expressed in adult mammalian kidney. So, it is likely that anti-HNF1 beta Rabbit Monoclonal antibody M01270 will work for WB with adult mammalian kidney.
Boster Scientific Support
Answered: 2017-11-21