Anti-HNF1 beta Rabbit Monoclonal Antibody

TCF-2/HNF-1 beta antibody

Boster Bio Anti-HNF1 beta Rabbit Monoclonal Antibody catalog # M01270. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01270
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-HNF1 beta Rabbit Monoclonal Antibody

View all TCF-2/HNF-1 beta Antibodies

SKU/Catalog Number

M01270

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-HNF1 beta Rabbit Monoclonal Antibody catalog # M01270. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HNF1 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01270)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HOH-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human HNF1 beta

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01270 is reactive to HNF1B in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

42 kDa

Calculated molecular weight

61324 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01270 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000

Positive Control

WB: Human kidney

Validation Images & Assay Conditions

Gene/Protein Information For HNF1B (Source: Uniprot.org, NCBI)

Gene Name

HNF1B

Full Name

Hepatocyte nuclear factor 1-beta

Weight

61324 MW

Superfamily

HNF1 homeobox family

Alternative Names

Hepatocyte nuclear factor 1-beta;HNF-1-beta;HNF-1B;Homeoprotein LFB3;Transcription factor 2;TCF-2;Variant hepatic nuclear factor 1;vHNF1;HNF1B;TCF2; HNF1B ADTKD3, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, RCAD, T2D, TCF-2, TCF2, VHNF1 HNF1 homeobox B hepatocyte nuclear factor 1-beta|HNF1 beta A|homeoprotein LFB3|transcription factor 2, hepatic

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HNF1B, check out the HNF1B Infographic

HNF1B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HNF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-HNF1 beta Rabbit Monoclonal Antibody

Question

Can you help my question with product M01270, anti-HNF1 beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01270 anti-HNF1 beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-15

Question

Is a blocking peptide available for product anti-HNF1 beta Rabbit Monoclonal antibody (M01270)?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

We do provide the blocking peptide for product anti-HNF1 beta Rabbit Monoclonal antibody (M01270). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-02

Question

I am looking for to test anti-HNF1 beta Rabbit Monoclonal antibody M01270 on mouse adult mammalian kidney for research purposes, then I may be interested in using anti-HNF1 beta Rabbit Monoclonal antibody M01270 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

The products we sell, including anti-HNF1 beta Rabbit Monoclonal antibody M01270, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-23

Question

Does anti-HNF1 beta Rabbit Monoclonal antibody M01270 work for WB with adult mammalian kidney?

Verified Customer

Verified customer

Asked: 2017-11-21

Answer

According to the expression profile of adult mammalian kidney, HNF1B is highly expressed in adult mammalian kidney. So, it is likely that anti-HNF1 beta Rabbit Monoclonal antibody M01270 will work for WB with adult mammalian kidney.

Boster Scientific Support

Answered: 2017-11-21

Order DetailsPrice
M01270

100μL

$370
M01270-30ul

30μL sample

$99
M01270-carrier-free-1ml

Carrier Free

$2590

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Free Secondary Antibody

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M01270
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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