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Product Info Summary
| SKU: | A04371-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-HMGCS2 Antibody (C-term)
SKU/Catalog Number
A04371-1
Size
80 µl
Description
Boster Bio Anti-HMGCS2 Antibody (C-term) (Catalog # A04371-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HMGCS2 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04371-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HMGCS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 478-508 amino acids from the C-terminal region of human HMGCS2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04371-1 is reactive to HMGCS2 in Human
Reconstitution
Calculated molecular weight
56635 Da
Background of HMGCS2
HMGCS2 belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04371-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from human pancreas, liver, testis and rat liver tissue lysate (from left to right), using HMGCS2 Antibody (C-term). A04371-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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HMGCS2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HMGCS2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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HMGCS2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For HMGCS2 (Source: Uniprot.org, NCBI)
Gene Name
HMGCS2
Full Name
Hydroxymethylglutaryl-CoA synthase, mitochondrial
Weight
56635 Da
Superfamily
thiolase-like superfamily
Alternative Names
Hydroxymethylglutaryl-CoA synthase, mitochondrial, HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS2 HMGCS2 3-hydroxy-3-methylglutaryl-CoA synthase 2 hydroxymethylglutaryl-CoA synthase, mitochondrial|3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial)|3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial)|HMG-CoA synthase|testicular tissue protein Li 88
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMGCS2, check out the HMGCS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMGCS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HMGCS2 Antibody (C-term) (A04371-1)
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