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Product Info Summary
| SKU: | A05313-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-HMGCS1 Antibody (Center)
SKU/Catalog Number
A05313-1
Size
80 µl
Description
Boster Bio Anti-HMGCS1 Antibody (Center) (Catalog # A05313-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HMGCS1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05313-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This HMGCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-317 amino acids from the Central region of human HMGCS1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05313-1 is reactive to HMGCS1 in Human, Mouse
Reconstitution
Calculated molecular weight
57294 Da
Background of HMGCS1
HMGCS1 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05313-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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HMGCS1 Antibody (Center) western blot analysis in U87-MG cell line,mouse liver and rat liver lysates (35ug/lane).This demonstrates the HMGCS1 antibody detected the HMGCS1 protein (arrow).
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of hela cells using HMGCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For HMGCS1 (Source: Uniprot.org, NCBI)
Gene Name
HMGCS1
Full Name
Hydroxymethylglutaryl-CoA synthase, cytoplasmic
Weight
57294 Da
Superfamily
thiolase-like superfamily
Alternative Names
Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS1, HMGCS HMGCS1 HMGCS 3-hydroxy-3-methylglutaryl-CoA synthase 1 hydroxymethylglutaryl-CoA synthase, cytoplasmic|3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase|3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble)|3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HMGCS1, check out the HMGCS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HMGCS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HMGCS1 Antibody (Center) (A05313-1)
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