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Product Info Summary
| SKU: | M12477-25 |
|---|---|
| Size: | 100 µl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H3 (acetyl K9) Rabbit Monoclonal Antibody
SKU/Catalog Number
M12477-25
Size
100 µl/vial
Form
Liquid
Description
Boster Bio Anti-Histone H3 (acetyl K9) Rabbit Monoclonal Antibody catalog # M12477-25. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H3 (acetyl K9) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12477-25)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
32H23
Isotype
IgG
Immunogen
A synthesized peptide derived from human Histone H3 (acetyl K9)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12477-25 is reactive to HIST1H3A in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
15.404kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12477-25 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of human breast tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of Histone H3 (acetyl K9) expression in C6 cell lysate treated with Trichostatin A.
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Figure 2. IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Click image to see more details
Figure 3. IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Click image to see more details
Figure 4. IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody (M12477-25).
Histone H3 (acetyl K9) was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Histone H3 (acetyl K9) Antibody (M12477-25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HIST1H3A (Source: Uniprot.org, NCBI)
Gene Name
HIST1H3A
Full Name
Histone H3.1
Weight
15.404kDa
Superfamily
histone H3 family
Alternative Names
Histone H3.1 H3C1 H3/A0, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3C1 H3 clustered histone 1 histone H3.1|H3 histone family, member A|histone 1, H3a|histone H3/a|histone cluster 1 H3 family member a|histone cluster 1, H3a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H3A, check out the HIST1H3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H3 (acetyl K9) Rabbit Monoclonal Antibody (M12477-25)
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