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Product Info Summary
| SKU: | M01371 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-HIPK2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01371
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-HIPK2 Rabbit Monoclonal Antibody catalog # M01371. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIPK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01371)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
21H33
Isotype
IgG
Immunogen
A synthesized peptide derived from human HIPK2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01371 is reactive to HIPK2 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
65569 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01371 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:30
FC 1:500
Positive Control
WB: human HT-1080 whole cell, human Raji whole cell, human PC-3 whole cell, human HEL whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HIPK2 using anti-HIPK2 antibody (M01371).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT-1080 whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIPK2 antigen affinity purified monoclonal antibody (Catalog # M01371) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIPK2 at approximately 100 kDa. The expected band size for HIPK2 is at 130 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HIPK2 (Source: Uniprot.org, NCBI)
Gene Name
HIPK2
Full Name
Homeodomain-interacting protein kinase 2
Weight
65569 MW
Superfamily
protein kinase superfamily
Alternative Names
Cell division control protein 45 homolog;PORC-PI-1;CDC45;CDC45L, CDC45L2;UNQ374/PRO710; Hipk2|1110014O20Rik, B230339E18Rik, St, Stank|homeodomain interacting protein kinase 2|homeodomain-interacting protein kinase 2|nuclear body-associated kinase 1|sialophorin tail-associated nuclear serine/threonine-protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIPK2, check out the HIPK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIPK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIPK2 Rabbit Monoclonal Antibody (M01371)
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