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Product Info Summary
| SKU: | M01248 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-HIF-2 alpha EPAS1 Rabbit Monoclonal Antibody
View all HIF-2 alpha/EPAS1 Antibodies
SKU/Catalog Number
M01248
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HIF-2 alpha EPAS1 Rabbit Monoclonal Antibody catalog # M01248. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF-2 alpha EPAS1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01248)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADD-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HIF-2 alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01248 is reactive to EPAS1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated molecular weight
96459 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01248 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of HIF-2 alpha in (1)MCF-7 cell lysate;(2)HeLa cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For EPAS1 (Source: Uniprot.org, NCBI)
Gene Name
EPAS1
Full Name
Endothelial PAS domain-containing protein 1
Weight
96459 MW
Alternative Names
Endothelial PAS domain-containing protein 1;EPAS-1;Basic-helix-loop-helix-PAS protein MOP2;Class E basic helix-loop-helix protein 73;bHLHe73;HIF-1-alpha-like factor;HLF;Hypoxia-inducible factor 2-alpha;HIF-2-alpha;HIF2-alpha;Member of PAS protein 2;PAS domain-containing protein 2;EPAS1;BHLHE73, HIF2A, MOP2, PASD2; EPAS1 ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73 endothelial PAS domain protein 1 endothelial PAS domain-containing protein 1|EPAS-1|HIF-1-alpha-like factor|HIF-1alpha-like factor|HIF-2-alpha|HIF2-alpha|PAS domain-containing protein 2|basic-helix-loop-helix-PAS protein MOP2|class E basic helix-loop-helix protein 73|hypoxia-inducible factor 2 alpha|member of PAS protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPAS1, check out the EPAS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPAS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF-2 alpha EPAS1 Rabbit Monoclonal Antibody (M01248)
Hello CJ!
M01248 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Proper autophagy is indispensable for angiogenesis during chick embryo development
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