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Product Info Summary
| SKU: | A01248 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-HIF-2 Alpha EPAS1 Antibody
View all HIF-2 alpha/EPAS1 Antibodies
SKU/Catalog Number
A01248
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HIF-2 Alpha EPAS1 Antibody catalog # A01248. Tested in WB,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF-2 Alpha EPAS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01248)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01248 is reactive to EPAS1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
96459 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01248 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of EPAS1 using Anti-HIF-2 Alpha EPAS1 Antibody (A01248).
Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-HIF-2 Alpha EPAS1 Antibody A01248
Western blot analysis of HIF-2 alpha on SiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EPAS1 (Source: Uniprot.org, NCBI)
Gene Name
EPAS1
Full Name
Endothelial PAS domain-containing protein 1
Weight
96459 MW
Alternative Names
Endothelial PAS domain-containing protein 1;EPAS-1;Basic-helix-loop-helix-PAS protein MOP2;Class E basic helix-loop-helix protein 73;bHLHe73;HIF-1-alpha-like factor;HLF;Hypoxia-inducible factor 2-alpha;HIF-2-alpha;HIF2-alpha;Member of PAS protein 2;PAS domain-containing protein 2;EPAS1;BHLHE73, HIF2A, MOP2, PASD2; EPAS1 ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73 endothelial PAS domain protein 1 endothelial PAS domain-containing protein 1|EPAS-1|HIF-1-alpha-like factor|HIF-1alpha-like factor|HIF-2-alpha|HIF2-alpha|PAS domain-containing protein 2|basic-helix-loop-helix-PAS protein MOP2|class E basic helix-loop-helix protein 73|hypoxia-inducible factor 2 alpha|member of PAS protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPAS1, check out the EPAS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPAS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF-2 Alpha EPAS1 Antibody (A01248)
Hello CJ!
A01248 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang P,Meng QQ,Wang W,Zhang S,Xiong X,Qin S,Zhang J,Li A,Liu Z. Icariin inhibits the inflammation through down-regulating NF-κB/HIF-2α signal pathways in chondrocytes. Biosci Rep.2020 Nov 6:BSR20203107.doi:10.1042 /BSR 20203 107.Epub ahead of print.PMID:3
Species: Mouse
A01248 usage in article: APP:IHC, SAMPLE:KNEE JOINTS, DILUTION:1:50
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