Anti-HIC5/TGFB1I1 Antibody Picoband™

Product Name

Anti-HIC5/TGFB1I1 Antibody Picoband™

View all HIC5/TGFB1I1 Antibodies

SKU/Catalog Number

A04630-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HIC5/TGFB1I1 Antibody Picoband™ catalog # A04630-2. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-HIC5/TGFB1I1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04630-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HIC5/TGFB1I1 recombinant protein (Position: E268-A447).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04630-2 is reactive to TGFB1I1 in Human, Monkey

Applications

A04630-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

Background of HIC5/TGFB1I1

Transforming growth factor beta-1-induced transcript 1 protein is a protein that in humans is encoded by the TGFB1I1 gene. This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Monkey
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of HIC5/TGFB1I1 using anti-HIC5/TGFB1I1 antibody (A04630-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIC5/TGFB1I1 antigen affinity purified polyclonal antibody (Catalog # A04630-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIC5/TGFB1I1 at approximately 75 kDa. The expected band size for HIC5/TGFB1I1 is at 75 kDa.

Gene/Protein Information For TGFB1I1 (Source: Uniprot.org, NCBI)

Alternative Names

Androgen receptor coactivator 55 kDa protein; androgen receptor coactivator ARA55; Androgen receptor-associated protein of 55 kDa; ARA55; ARA55Hydrogen peroxide-inducible clone 5 protein; HIC5; HIC-5; TGFB1I1; transforming growth factor beta 1 induced transcript 1; transforming growth factor beta-1-induced transcript 1 protein; TSC-5 TGFB1I1 ARA55, HIC-5, HIC5, TSC-5 transforming growth factor beta 1 induced transcript 1 transforming growth factor beta-1-induced transcript 1 protein|androgen receptor coactivator 55 kDa protein|androgen receptor coactivator ARA55|androgen receptor-associated protein of 55 kDa|hydrogen peroxide-inducible clone 5 protein|hydrogen peroxide-inducible clone-5

For more info on TGFB1I1, check out the TGFB1I1 Infographic

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In this infographic, you will see the following information for TGFB1I1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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