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Product Info Summary
| SKU: | A00506 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-HFE Monoclonal Antibody
SKU/Catalog Number
A00506
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HFE Monoclonal Antibody catalog # A00506. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HFE Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00506)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3F1
Isotype
IgG
Immunogen
Purified recombinant fragment of human HFE expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00506 is reactive to HFE in Human
Applications
A00506 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
40108 MW
Background of HFE
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion
Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.
Turner, M. et al. (2000) Immunol. Today 21, 148-154.
Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IF, 1:200-1:1000
Validation Images & Assay Conditions
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Immunofluorescence (IF) analysis of HepG2 cells using HFE Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
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Western Blot (WB) analysis using HFE Monoclonal antibody against HEK293 (1) and HFE-hIgGFc transfected HEK293 (2) cell lysate.
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ELISA analysis of HFE antibody.
Protein Target Info & Infographic
Gene/Protein Information For HFE (Source: Uniprot.org, NCBI)
Gene Name
HFE
Full Name
Hereditary hemochromatosis protein
Weight
40108 MW
Superfamily
MHC class I family
Alternative Names
dJ221C16.10.1; hemochromatosis; hereditary hemochromatosis protein HLA-H; hereditary hemochromatosis protein; HH; high Fe; HLAH; HLA-HHFE1; MGC103790; MHC class I-like protein HFE; MVCD7 HFE HFE1, HH, HLA-H, MVCD7, TFQTL2 homeostatic iron regulator hereditary hemochromatosis protein|MHC class I-like protein HFE|hereditary hemochromatosis protein HLA-H|high Fe
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HFE, check out the HFE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HFE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HFE Monoclonal Antibody (A00506)
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No publications found for A00506
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