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Product Info Summary
| SKU: | A00539 |
|---|---|
| Size: | 250ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-HER3 ERBB3 Antibody
View all ErbB3/Her3 Antibodies
SKU/Catalog Number
A00539
Size
250ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-HER3 ERBB3 Antibody (Catalog # A00539). Tested in ELISA, IP, WB applications. This antibody reacts with Human.
Storage & Handling
Store Anti-HER3 at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-HER3 ERBB3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00539)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-HER3 whole rabbit serum was prepared by repeated immunizations with a HER3 fusion protein corresponding to amino acids 1283 to 1323 (40 amino acids) at the carboxy-terminus of human HER3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00539 is reactive to ERBB3 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
24607 MW
Background of ErbB3/Her3
HER3 antibody is ideal for western blotting, ELISA and IP. The human Epidermal Growth Factor Receptor (EGFR) family is a family of receptor tyrosine kinases consisting of 4 members: EGFR, HER2, HER3 and HER4. These receptors are expressed on a wide variety of tissue types and have been implicated in many human cancers, including breast, gastric, colon and prostate. Strong expression of both HER3 and HER2 (also known as p185neu) by tumor cells can be associated with poor outcome for patients. The ligand for HER3 and HER4 is called Neu Differentiation Factor (NDF) or Heregulin. In some instances, antibodies against these receptors prove effective in combination therapy regimens for treatment of the disease. Signaling through the EGFR family involves the combinatorial dimerization of the four known family members and is mediated by at least 8 different hormones, including EGF and the heregulins. Heregulin binding and tyrosine phosphorylation by HER3 or HER4 is enhanced in the presence of HER2. Structurally the members of the EGFR family are 1210 to 1342 amino acids in length with approximately 650 amino acids of the extracellular domain being heavily glycosylated. The extracellular domains show up to 70% sequence homology.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00539 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IP: Use 5 µL antibody.
WB: 1:500- 1:2,000
Validation Images & Assay Conditions
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Signaling pathways activated by the ErB3/HER3 Receptor.
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Figure 1. Western blot analysis of ERBB3 using anti-ERBB3 antibody (A00539).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (Catalog # A00539) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ERBB3.
Protein Target Info & Infographic
Gene/Protein Information For ERBB3 (Source: Uniprot.org, NCBI)
Gene Name
ERBB3
Full Name
Receptor tyrosine-protein kinase erbB-3
Weight
24607 MW
Superfamily
protein kinase superfamily
Alternative Names
HER3, HER, c erbB 3, c erbB3, ERBB3, ERBB3 protein, erbB3 S, Glial growth factor receptor ERBB3 ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 erb-b2 receptor tyrosine kinase 3 receptor tyrosine-protein kinase erbB-3|human epidermal growth factor receptor 3|proto-oncogene-like protein c-ErbB-3|tyrosine kinase-type cell surface receptor HER3|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERBB3, check out the ERBB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HER3 ERBB3 Antibody (A00539)
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Customer Q&As
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15 Customer Q&As for Anti-HER3 ERBB3 Antibody
Question
I was wanting to use your anti-HER3 antibody for IP for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
As indicated on the product datasheet, A00539 anti-HER3 antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-28
Question
We are currently using anti-HER3 antibody A00539 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The anti-HER3 antibody (A00539) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-24
Question
I see that the anti-HER3 antibody A00539 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-02
Question
We have tried in the past anti-HER3 antibody for IP on placenta small intestine in the past. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining placenta small intestine as well as placenta in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
N. Banerjee
Verified customer
Asked: 2019-09-10
Answer
I have checked the website and datasheets of our anti-HER3 antibody and it seems that A00539 has been tested on mouse in both IP and WB. Thus A00539 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-10
Question
Can you help my question with product A00539, anti-HER3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00539 anti-HER3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-28
Question
Our lab were happy with the WB result of your anti-HER3 antibody. However we have been able to see positive staining in corpus callosum isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ERBB3 supposed to be expressed?
B. Johnson
Verified customer
Asked: 2019-07-18
Answer
From literature, corpus callosum does express ERBB3. Generally ERBB3 expresses in isoform 1: cell membrane. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-18
Question
Will anti-HER3 antibody A00539 work for IP with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
According to the expression profile of cervix carcinoma erythroleukemia, ERBB3 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-HER3 antibody A00539 will work for IP with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-07-10
Question
Is a blocking peptide available for product anti-HER3 antibody (A00539)?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We do provide the blocking peptide for product anti-HER3 antibody (A00539). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-27
Question
I am interested in to test anti-HER3 antibody A00539 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-HER3 antibody A00539 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
The products we sell, including anti-HER3 antibody A00539, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-17
Question
Does A00539 anti-HER3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
You can see on the product datasheet, A00539 anti-HER3 antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-27
Question
Is this A00539 anti-HER3 antibody reactive to the isotypes of ERBB3?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
The immunogen of A00539 anti-HER3 antibody is anti-HER3 whole rabbit serum was prepared by repeated immunizations with a HER3 fusion protein corresponding to amino acids 1283 to 1323 (40 amino acids) at the carboxy-terminus of human HER3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-15
Question
We have been able to see staining in rat hippocampus. Do you have any suggestions? Is anti-HER3 antibody supposed to stain hippocampus positively?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
From what I have seen in literature hippocampus does express ERBB3. From what I have seen in Uniprot.org, ERBB3 is expressed in corpus callosum, placenta, hippocampus, placenta small intestine, skin, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-08-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-HER3 antibody A00539. Let me know if you need anything else.
N. Parker
Verified customer
Asked: 2015-09-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-29
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-HER3 antibody A00539. Please let me know if you require anything else.
N. Bhatt
Verified customer
Asked: 2015-07-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-09
Question
Do you have a BSA free version of anti-HER3 antibody A00539 available?
S. Krishna
Verified customer
Asked: 2013-08-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HER3 antibody A00539 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-08-23
