Anti-HELLS Antibody Picoband™

Product Name

Anti-HELLS Antibody Picoband™

View all SMARCA6 Antibodies

SKU/Catalog Number

A04702-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-HELLS Antibody Picoband™ catalog # A04702-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-HELLS Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04702-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human HELLS recombinant protein (Position: N167-F838). Human HELLS shares 93% amino acid (aa) sequence identity with mouse HELLS.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04702-1 is reactive to HELLS in Human, Mouse

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

97 kDa

Calculated molecular weight

52588 MW

Background of SMARCA6

Lymphoid-specific helicase (Lsh) is a member of the SNF2 helicase family of chromatin remodeling proteins that in humans is encoded by the HELLS gene. This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04702-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HELLS using anti-HELLS antibody (A04702-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HELLS antigen affinity purified polyclonal antibody (Catalog # A04702-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HELLS at approximately 97 kDa. The expected band size for HELLS is at 97 kDa.

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Figure 2. IF analysis of HELLS using anti-HELLS antibody (A04702-1) and anti-Beta Tubulin antibody (M01857-3).
HELLS was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HELLS Antibody (A04702-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-HELLS antibody (A04702-1).
Overlay histogram showing PC-3 cells stained with A04702-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HELLS Antibody (A04702-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For HELLS (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.6.1; EC 3.6.4.-; helicase, lymphoid-specific; LSHSWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin; Nbla10143; PASGFLJ10339; Proliferation-associated SNF2-like protein; SMARCA6lymphoid-specific helicase; subfamily A, member 6; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 6 HELLS ICF4, LSH, Nbla10143, PASG, SMARCA6 helicase, lymphoid specific lymphoid-specific helicase|SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6|proliferation-associated SNF2-like protein

For more info on HELLS, check out the HELLS Infographic

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In this infographic, you will see the following information for HELLS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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