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Product Info Summary
| SKU: | A02592-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-HEB/TCF12 Antibody Picoband®
View all TCF-12/HTF4 Antibodies
SKU/Catalog Number
A02592-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HEB/TCF12 Antibody Picoband® catalog # A02592-3. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HEB/TCF12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02592-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human HEB/TCF12 recombinant protein (Position: S67-G300). Human TCF12 shares 96.6% amino acid (aa) sequence identity with both mouse and rat TCF12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02592-3 is reactive to TCF12 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
85 kDa
Calculated molecular weight
73 kDa
Background of TCF-12/HTF4
Transcription factor 12 is a protein that in humans is encoded by the TCF12 gene. The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02592-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A549 whole cell, human Jurkat whole cell, human Hela whole cell, human 293T whole cell, rat thymus tissue, rat C6 whole cell, mouse thymus tissue, mouse NIH/3T3 whole cell
FCM: 293T cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HEB/TCF12 using anti-HEB/TCF12 antibody (A02592-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEB/TCF12 antigen affinity purified polyclonal antibody (Catalog # A02592-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEB/TCF12 at approximately 85 kDa. The expected band size for HEB/TCF12 is at 73 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-HEB/TCF12 antibody (A02592-3).
Overlay histogram showing 293T cells stained with A02592-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HEB/TCF12 Antibody (A02592-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TCF12 (Source: Uniprot.org, NCBI)
Gene Name
TCF12
Full Name
Transcription factor 12
Weight
73 kDa
Alternative Names
BHLHB20; bHLHb20E-box-binding protein; Class B basic helix-loop-helix protein 20; HEB; HEBDNA-binding protein HTF4; helix-loop-helix transcription factor 4; HsT17266; HTF4; HTF4TCF-12; TCF12; TCF-12; transcription factor 12; Transcription factor HTF-4 TCF12 CRS3, HEB, HTF4, HsT17266, TCF-12, bHLHb20, p64 transcription factor 12 transcription factor 12|DNA-binding protein HTF4|E-box-binding protein|class B basic helix-loop-helix protein 20|helix-loop-helix transcription factor 4|transcription factor HTF-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TCF12, check out the TCF12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TCF12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HEB/TCF12 Antibody Picoband® (A02592-3)
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