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Product Info Summary
| SKU: | A02697-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-heavy chain Myosin/Myh3 Antibody Picoband®
View all Myosin Heavy Chain 3 Antibodies
SKU/Catalog Number
A02697-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-heavy chain Myosin/Myh3 Antibody Picoband® catalog # A02697-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-heavy chain Myosin/Myh3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02697-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat heavy chain Myosin/Myh3 recombinant protein (Position: K44-S119).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02697-1 is reactive to Myh3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
224 kDa
Calculated molecular weight
91791 MW
Background of Myosin Heavy Chain 3
Myosin-3 is a protein that in humans is encoded by the MYH3 gene. Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02697-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells,Human, Rat
ELISA, 0.1-0.5 μg/ml, Rat
Positive Control
WB: rat heart tissue
IHC: mouse cardiac tissue, rat cardiac tissue
FCM: U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of heavy chain Myosin/Myh3 using anti-heavy chain Myosin/Myh3 antibody (A02697-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-heavy chain Myosin/Myh3 antigen affinity purified polyclonal antibody (Catalog # A02697-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for heavy chain Myosin/Myh3 at approximately 224 kDa. The expected band size for heavy chain Myosin/Myh3 is at 224 kDa.
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Figure 2. IHC analysis of heavy chain Myosin/Myh3 using anti-heavy chain Myosin/Myh3 antibody (A02697-1).
heavy chain Myosin/Myh3 was detected in a paraffin-embedded section of mouse cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-heavy chain Myosin/Myh3 Antibody (A02697-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of heavy chain Myosin/Myh3 using anti-heavy chain Myosin/Myh3 antibody (A02697-1).
heavy chain Myosin/Myh3 was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-heavy chain Myosin/Myh3 Antibody (A02697-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of U20S cells using anti-heavy chain Myosin/Myh3 antibody (A02697-1).
Overlay histogram showing U20S cells stained with A02697-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-heavy chain Myosin/Myh3 Antibody (A02697-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Myh3 (Source: Uniprot.org, NCBI)
Gene Name
Myh3
Full Name
Myosin-3
Weight
91791 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Neuronal PAS domain-containing protein 2;Neuronal PAS2;Basic-helix-loop-helix-PAS protein MOP4;Class E basic helix-loop-helix protein 9;bHLHe9;Member of PAS protein 4;PAS domain-containing protein 4;NPAS2;BHLHE9, MOP4, PASD4; MYH3 CPSFS1A, CPSFS1B, CPSKF1A, CPSKF1B, DA2A, DA2B, DA2B3, DA8, HEMHC, MYHC-EMB, MYHSE1, SMHCE myosin heavy chain 3 myosin-3|myosin heavy chain, fast skeletal muscle, embryonic|myosin, heavy chain 3, skeletal muscle, embryonic|myosin, heavy polypeptide 3, skeletal muscle, embryonic|myosin, skeletal, heavy chain, embryonic 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Myh3, check out the Myh3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Myh3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-heavy chain Myosin/Myh3 Antibody Picoband® (A02697-1)
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