Myosin-3 Antibodies

Myosin-3 (MYH3)

Muscle contraction. .

Gene Information

Human
Gene Name:	MYH3
Uniprot:	P11055
Entrez:	4621

Family

Belongs to:
TRAFAC class myosin-kinesin ATPase superfamily

Alternative Names

HEMHC; Muscle embryonic myosin heavy chain; MYHC-EMB; MYHSE1; Myosin heavy chain, fast skeletal muscle, embryonic; myosin, heavy chain 3, skeletal muscle, embryonic; myosin, heavy polypeptide 3, skeletal muscle, embryonic; myosin, skeletal, heavy chain, embryonic 1; myosin-3; SMHCEMyosin heavy chain 3

Molecular Weight

Mass (kDA):
223.905 kDA

Genomic Context

Human
Location:	17p13.1
Sequence:	17; NC_000017.11 (10628527..10678347, complement)

Tissue Specificity

Expressed in fetal bone, thymus, placenta, heart, brain, and liver.

Subcellular Localizations

Cytoplasm, myofibril. Thick filaments of the myofibrils.

Diseases

• Arthrogryposis
• Distal Arthrogryposis Syndrome
• Muscle Contracture
• Freeman-sheldon Syndrome
• Arthrogryposis, Distal, Type 2b
• Congenital Clubfoot
• Myopathy
• Congenital Camptodactyly
• Neoplasms
• Ophthalmoplegia
• Muscle Weakness
• Distal Muscular Dystrophies

• Weakness
• Welander Distal Myopathy
• Vertical Talus
• Uterine Prolapse
• Fibrosis
• External Ophthalmoplegia
• Congenital Abnormality

Pathways

• Regeneration
• Muscle Contraction
• Cell Differentiation
• Cell Growth
• Regulation Of Cell Growth
• Myoblast Differentiation
• Transport
• Striated Muscle Contraction
• Cell Activation
• General Adaptation Syndrome
• Fibril Organization
• Response To Stress
• Cell Migration

• Satellite Cell Activation
• Muscle Adaptation
• Localization
• Cell Division
• Tissue Development
• Cell Cycle
• Skeletal Muscle Contraction

PTMs

• Phosphorylation

Research Areas

• Dendritic Cell Markers
• Immunology

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MYH3

Boster Bio - Best Uses Of The MYH3 Marker

Muscle fibers with Myosin-3 are a major source of energy. Boster Bio is a powerful marker for assessing the health of these fibers. The MYH3 Marker can help scientists determine which species and applications contain high levels of this protein. They can also submit their results for product credits. Scientists all over the world can take advantage of this tool and benefit from it.

Muscle fibers containing myosin-3

The MYH3 gene provides instructions for making myosin-3, a protein that is involved in movement and transport within the cell. Myosins are proteins that are found in muscle fibers, which are primarily composed of thick filaments of myosin and thin filaments of actin. The two types of filaments interact with one another to allow the muscles to contract and move. Muscle fibers express myosin during the early development stage.

The MYH3 marker can be used for cell-free analysis of myofibrils, single fibers, and isolated myosin S1 to characterize their kinetic parameters. This marker can also be used for studies on the regulation of protein-protein interactions and can address the question of why myosin isoforms are needed during muscle development. There are many potential uses of this gene, and it is an important marker in this field.

The MYH3 gene can be used in muscle disease models, such as those caused by chronic electrical stimulation or denervation. Myosin is expressed in skeletal muscle in animal models of muscle diseases, and it can be used to help detect whether the affected muscles are actually regenerating. For example, it can also be used to diagnose Duchenne muscular dystrophy in the mdx mouse model.

Developmental myosins are expressed in both neonatal and embryonic stages of development. MyHC-emb and -neo are found in the skeletal muscle of the rat several days after birth, respectively. This switch from neonatal myosin to adult myosin isoforms is regulated by MyoD and Myf5 genes.

The MYH3 gene is expressed in different skeletal muscles. The transition from the embryonic to the adult fast myosin isoform is very different in the pectoralis muscle. The other muscles are predominantly neonatal. The MYHC gene is expressed in all three stages of muscle regeneration. There are several applications of the MYH3 gene in muscle and are discussed below.

Actin Alpha Cardiac Muscle 1 (ACTC1) is a myogenic gene expressed in the developing heart and skeletal muscles. It is expressed in both the neonatal and fetal heart. The MYHC gene is expressed in cardiac muscle. The MYH3 gene can be used to identify these cells and other types of muscle in different tissues. It is not known whether the MYHC gene is required for myofibrillogenesis.

The MYH3 gene is part of a cluster of genes containing MYH2, MYH3, MYH2, and MYH4-MYh23. It is thought that ACTC1 overexpression promotes the formation of myotubes in the adult heart. However, the MYHC-ACTC1 gene overexpression inhibits the growth of myotubes in adult brain.

Benefits of using the MYH3 Marker

The MYH3 marker is a quantitative trait gene. The markers were validated and used to identify the MYH3 allele in 18 F7 pigs. The expression of MYH3 protein was measured by western blot analysis using RIPA buffer and BCA protein assay kit. After centrifugation at 4degC for 15 min, the total protein was separated using SDS-PAGE.

Cho et al., who performed transient transfection analysis on porcine fibroblast cells, found that the MYH3 q variant functions as a weaker repressor element. This leads to increased MYH3 transcription in skeletal muscle of KNPs and Q allele carriers. In the same study, the 6-bp deletion variant did not affect the protein level or the transcriptional activity of luciferase reporter constructs.