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Product Info Summary
| SKU: | M00839 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HDAC3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00839
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC3 Rabbit Monoclonal Antibody catalog # M00839. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00839)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EFH-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00839 is reactive to HDAC3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
48848 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00839 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:200
IP 1:30
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of HDAC3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For HDAC3 (Source: Uniprot.org, NCBI)
Gene Name
HDAC3
Full Name
Histone deacetylase 3
Weight
48848 MW
Superfamily
histone deacetylase family
Alternative Names
Histone deacetylase 3;HD3;3.5.1.98;RPD3-2;SMAP45;HDAC3; HDAC3 HD3, KDAC3, RPD3, RPD3-2 histone deacetylase 3 histone deacetylase 3|SMAP45
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC3, check out the HDAC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC3 Rabbit Monoclonal Antibody (M00839)
Hello CJ!
M00839 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Interleukin-18 down-regulates multidrug resistance-associated protein 2 expression through farnesoid x receptor associated with nuclear factor kappa B and %u2026
Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in hippocampal neuronal death following oxygen-glucose deprivation
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Customer Q&As
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16 Customer Q&As for Anti-HDAC3 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-HDAC3 Rabbit Monoclonal antibody for IF for mouse fibroblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse fibroblast identification?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
As indicated on the product datasheet, M00839 anti-HDAC3 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse fibroblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-23
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-HDAC3 Rabbit Monoclonal antibody M00839. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
My question regarding product M00839, anti-HDAC3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00839 anti-HDAC3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-06
Question
Is a blocking peptide available for product anti-HDAC3 Rabbit Monoclonal antibody (M00839)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We do provide the blocking peptide for product anti-HDAC3 Rabbit Monoclonal antibody (M00839). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-13
Question
Do you have a BSA free version of anti-HDAC3 Rabbit Monoclonal antibody M00839 available?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HDAC3 Rabbit Monoclonal antibody M00839 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-31
Question
Please see the WB image, lot number and protocol we used for fibroblast using anti-HDAC3 Rabbit Monoclonal antibody M00839. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-15
Question
My team were well pleased with the WB result of your anti-HDAC3 Rabbit Monoclonal antibody. However we have observed positive staining in skin nucleus. using this antibody. Is that expected? Could you tell me where is HDAC3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
From literature, skin does express HDAC3. Generally HDAC3 expresses in nucleus. Regarding which tissues have HDAC3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983
Erythroleukemia, Pubmed ID: 23186163
Fibroblast, Pubmed ID: 9346952
Leukemic T-cell, Pubmed ID: 19690332
Skin, Pubmed ID: 15489334
Spleen, and T-cell, Pubmed ID: 9464271
Boster Scientific Support
Answered: 2019-10-07
Question
Will anti-HDAC3 Rabbit Monoclonal antibody M00839 work for IF with fibroblast?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
According to the expression profile of fibroblast, HDAC3 is highly expressed in fibroblast. So, it is likely that anti-HDAC3 Rabbit Monoclonal antibody M00839 will work for IF with fibroblast.
Boster Scientific Support
Answered: 2019-08-02
Question
I see that the anti-HDAC3 Rabbit Monoclonal antibody M00839 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-18
Question
Does M00839 anti-HDAC3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Patel
Verified customer
Asked: 2019-05-15
Answer
You can see on the product datasheet, M00839 anti-HDAC3 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-15
Question
Is this M00839 anti-HDAC3 Rabbit Monoclonal antibody reactive to the isotypes of HDAC3?
H. Taylor
Verified customer
Asked: 2018-12-31
Answer
The immunogen of M00839 anti-HDAC3 Rabbit Monoclonal antibody is A synthesized peptide derived from human HDAC3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-31
Question
We want using your anti-HDAC3 Rabbit Monoclonal antibody for circadian regulation of gene expression studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
We appreciate your inquiry. This M00839 anti-HDAC3 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-13
Question
We are currently using anti-HDAC3 Rabbit Monoclonal antibody M00839 for mouse tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
The anti-HDAC3 Rabbit Monoclonal antibody (M00839) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-12
Question
We ordered your anti-HDAC3 Rabbit Monoclonal antibody for IP on right hemisphere of cerebellum last year. I am using rat, and We want to use the antibody for IF next. We want examining right hemisphere of cerebellum as well as fibroblast in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Z. Zhao
Verified customer
Asked: 2015-06-23
Answer
I have checked the website and datasheets of our anti-HDAC3 Rabbit Monoclonal antibody and I see that M00839 has been validated on rat in both IP and IF. Thus M00839 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-06-23
Question
We have been able to see staining in mouse right hemisphere of cerebellum. Do you have any suggestions? Is anti-HDAC3 Rabbit Monoclonal antibody supposed to stain right hemisphere of cerebellum positively?
L. Johnson
Verified customer
Asked: 2015-03-19
Answer
From literature right hemisphere of cerebellum does express HDAC3. From Uniprot.org, HDAC3 is expressed in right hemisphere of cerebellum, spleen t-cell, fibroblast, skin, cervix carcinoma, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have HDAC3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983
Erythroleukemia, Pubmed ID: 23186163
Fibroblast, Pubmed ID: 9346952
Leukemic T-cell, Pubmed ID: 19690332
Skin, Pubmed ID: 15489334
Spleen, and T-cell, Pubmed ID: 9464271
Boster Scientific Support
Answered: 2015-03-19
Question
I was wanting to use to test anti-HDAC3 Rabbit Monoclonal antibody M00839 on mouse fibroblast for research purposes, then I may be interested in using anti-HDAC3 Rabbit Monoclonal antibody M00839 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Kulkarni
Verified customer
Asked: 2014-03-31
Answer
The products we sell, including anti-HDAC3 Rabbit Monoclonal antibody M00839, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-31
