Product Info Summary
SKU: | M05215 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-HDAC10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05215
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC10 Rabbit Monoclonal Antibody catalog # M05215. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05215)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HEO-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC10
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05215 is reactive to HDAC10 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated molecular weight
71445 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05215 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
ICC/IF: Hela cell
Validation Images & Assay Conditions
![m05215 wb7 m05215 wb7](https://www.bosterbio.com/media/catalog/product/m/0/m05215-wb7.jpg)
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
![m05215 wb m05215 wb](https://www.bosterbio.com/media/catalog/product/m/0/m05215-wb.jpg)
Click image to see more details
Western blot analysis of HDAC10 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
![m05215 if m05215 if](https://www.bosterbio.com/media/catalog/product/m/0/m05215-if.jpg)
Click image to see more details
Immunofluorescent analysis of Hela cells, using HDAC10 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HDAC10 (Source: Uniprot.org, NCBI)
Gene Name
HDAC10
Full Name
Polyamine deacetylase HDAC10
Weight
71445 MW
Superfamily
histone deacetylase family
Alternative Names
Histone deacetylase 10;HD10;3.5.1.98;HDAC10; HDAC10 HD10 histone deacetylase 10 polyamine deacetylase HDAC10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC10, check out the HDAC10 Infographic
![HDAC10 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC10 Rabbit Monoclonal Antibody (M05215)
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No publications found for M05215
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Customer Q&As
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5 Customer Q&As for Anti-HDAC10 Rabbit Monoclonal Antibody
Question
Is this M05215 anti-HDAC10 Rabbit Monoclonal antibody reactive to the isotypes of HDAC10?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The immunogen of M05215 anti-HDAC10 Rabbit Monoclonal antibody is A synthesized peptide derived from human HDAC10 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-06
Question
I would like to test anti-HDAC10 Rabbit Monoclonal antibody M05215 on human amygdala for research purposes, then I may be interested in using anti-HDAC10 Rabbit Monoclonal antibody M05215 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The products we sell, including anti-HDAC10 Rabbit Monoclonal antibody M05215, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-04
Question
Is a blocking peptide available for product anti-HDAC10 Rabbit Monoclonal antibody (M05215)?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
We do provide the blocking peptide for product anti-HDAC10 Rabbit Monoclonal antibody (M05215). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-06
Question
Does anti-HDAC10 Rabbit Monoclonal antibody M05215 work on dog WB with leukemia?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
Our lab technicians have not tested anti-HDAC10 Rabbit Monoclonal antibody M05215 on dog. You can run a BLAST between dog and the immunogen sequence of anti-HDAC10 Rabbit Monoclonal antibody M05215 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog leukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-28
Question
I see that the anti-HDAC10 Rabbit Monoclonal antibody M05215 works with IP, what is the protocol used to produce the result images on the product page?
S. Mitchell
Verified customer
Asked: 2017-07-05
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-05