Click image to see more details
Product Info Summary
| SKU: | PB9210 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HCN1 Antibody Picoband®
SKU/Catalog Number
PB9210
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HCN1 Antibody Picoband® catalog # PB9210. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HCN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9210)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HCN1 recombinant protein (Position: E618-L890). Human HCN1 shares 82% amino acid (aa) sequence identity with both mouse and rat HCN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9210 is reactive to HCN1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
98796 MW
Background of Hcn1
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, also known as HAC-2 or BCNG-1, is a protein that in humans is encoded by the HCN1 gene. It is mapped to 5p12. The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. It may mediates responses to sour stimuli.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9210 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human U87 whole cell, human SH-SY5Y whole cell, human U20S whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HCN1 using anti-HCN1 antibody (PB9210).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCN1 antigen affinity purified polyclonal antibody (Catalog # PB9210) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCN1 at approximately 120 kDa. The expected band size for HCN1 is at 99 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HCN1 (Source: Uniprot.org, NCBI)
Gene Name
HCN1
Full Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Weight
98796 MW
Superfamily
potassium channel HCN family
Alternative Names
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1;Brain cyclic nucleotide-gated channel 1;BCNG-1;HCN1;BCNG1; Hcn1|Bcng, Bcng1, C630013B14Rik, HAC, HAC2|hyperpolarization activated cyclic nucleotide gated potassium channel 1|potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1|BCNG-1|HAC-2|brain cyclic nucleotide-gated channel 1|hyperpolarization-activated cation channel 2|hyperpolarization-activated, cyclic nucleotide-gated K+ 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HCN1, check out the HCN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HCN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HCN1 Antibody Picoband® (PB9210)
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Customer Q&As
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7 Customer Q&As for Anti-HCN1 Antibody Picoband®
Question
I see that the anti-HCN1 antibody PB9210 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-17
Question
Does PB9210 anti-HCN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
You can see on the product datasheet, PB9210 anti-HCN1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-31
Question
I was wanting to use your anti-HCN1 antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
It shows on the product datasheet, PB9210 anti-HCN1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-24
Question
Is this PB9210 anti-HCN1 antibody reactive to the isotypes of HCN1?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The immunogen of PB9210 anti-HCN1 antibody is E.coli-derived human HCN1 recombinant protein (Position: E618-L890). Human HCN1 shares 82% amino acid (aa) sequence identity with both mouse and rat HCN1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-26
Question
We need to test anti-HCN1 antibody PB9210 on rat brain for research purposes, then I may be interested in using anti-HCN1 antibody PB9210 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Thomas
Verified customer
Asked: 2019-08-08
Answer
The products we sell, including anti-HCN1 antibody PB9210, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-08
Question
Is a blocking peptide available for product anti-HCN1 antibody (PB9210)?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We do provide the blocking peptide for product anti-HCN1 antibody (PB9210). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-07
Question
We are currently using anti-HCN1 antibody PB9210 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-HCN1 antibody (PB9210) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
