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Product Info Summary
| SKU: | A01120-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-hCG receptor/LHR/LHCGR Antibody Picoband®
SKU/Catalog Number
A01120-2
Size
100 μg/vial
Form
Lyophilized
Description
Rabbit IgG polyclonal antibody for hCG receptor/LHR/LHCGR detection. Tested with WB, ELISA in Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-hCG receptor/LHR/LHCGR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01120-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LHCGR recombinant protein (Position: Q67-N215).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01120-2 is reactive to LHCGR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
78.643kDa
Background of LHR
The luteinizing hormone/choriogonadotropin receptor is a member of a subfamily of G protein-coupled receptors (GPCR) characterized by the presence of a large N-terminal extracellular domain containing several leucine-rich repeats (LRR). This glycoprotein hormone receptor family has been named the LRR-containing GPCR (LGR) family. The LHCGR gene contains 11 exons and spans approximately 80 kb and it is mapped to chromosome 2p21. In the ovary, theca, stromal, late-stage (luteinizing) granulosa, and luteal cells contain LHCGR. In the testes, Leydig cells contain LHCRG.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01120-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of hCG receptor/LHR/LHCGR using anti-hCG receptor/LHR/LHCGR antibody (A01120-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hCG receptor/LHR/LHCGR antigen affinity purified polyclonal antibody (Catalog # A01120-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hCG receptor/LHR/LHCGR at approximately 78 kDa. The expected band size for hCG receptor/LHR/LHCGR is at 78 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LHCGR (Source: Uniprot.org, NCBI)
Gene Name
LHCGR
Full Name
Lutropin-choriogonadotropic hormone receptor
Weight
78.643kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
CREB-regulated transcription coactivator 2; Transducer of regulated cAMP response element-binding protein 2; TORC-2; Transducer of CREB protein 2; CRTC2; TORC2 LHCGR HHG, LCGR, LGR2, LH/CG-R, LH/CGR, LHR, LHRHR, LSH-R, ULG5 luteinizing hormone/choriogonadotropin receptor lutropin-choriogonadotropic hormone receptor|hypergonadotropic hypogonadism|lutropin/choriogonadotropin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LHCGR, check out the LHCGR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LHCGR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hCG receptor/LHR/LHCGR Antibody Picoband® (A01120-2)
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