Anti-HAP1 Antibody

Product Name

Anti-HAP1 Antibody

View all HAP1 Antibodies

SKU/Catalog Number

A01658

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-HAP1 Antibody (Catalog # A01658). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

HAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-HAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01658)

Host

Rabbit

Contents

HAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

HAP1 antibody was raised against a 19 amino acid synthetic peptide from near the center of human HAP1. The immunogen is located within amino acids 310 - 360 of HAP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of TYK2 are known to exist; this antibody only detects the two longest isoforms.

Reactive Species

A01658 is reactive to HAP1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

75506 MW

Background of HAP1

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. HAP1 was initially identified through a two-hybrid library screening; the binding of HAP1 to huntingtin correlated with the expansion of the polyglutamine tract. HAP1 also interacts with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), suggesting that HAP1 may play a role in vesicular trafficking or organelle transport. HAP1 is also involved with the huntingtin-enhanced BDNF transport along the cellular microtubles. Attenuation of this process led to the loss of neurotrophic support and neuronal toxicity, which suggests that loss of this function might contribute to pathogenesis. Several alternatively spliced isoforms have been described for HAP1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01658 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

HAP1 antibody can be used for detection of HAP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For HAP1 (Source: Uniprot.org, NCBI)

Alternative Names

HLP, HAP2, HIP5, hHLP1, HLP1, Huntingtin-associated protein 1, Neuroan 1, HAP-1 HAP1 HAP2, HIP5, HLP, hHLP1 huntingtin associated protein 1 huntingtin-associated protein 1|HAP-1|epididymis secretory sperm binding protein|huntingtin-associated protein 2|neuroan 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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