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Product Info Summary
| SKU: | M06819-30 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-H3f3b Antibody (C-Term)
SKU/Catalog Number
M06819-30
Size
50 µl
Description
Boster Bio Anti-H3f3b Antibody (C-Term) (Catalog # M06819-30). Tested in WB application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-H3f3b Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-30)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This H3f3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 103-136 amino acids from mouse H3f3b.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06819-30 is reactive to H3-3a in Mouse
Reconstitution
Calculated molecular weight
15328 Da
Background of H3-3A
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06819-30 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-H3f3b Antibody (C-Term) at 1:8000 dilution
Lane 1: NIH/3T3 whole cell lysate
Lane 2: mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-H3f3b Antibody (C-Term) at 1:2000 dilution
Lane 1: mouse cerebellum lysate
Lane 2: mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For H3-3a (Source: Uniprot.org, NCBI)
Gene Name
H3-3a
Full Name
Histone H3.3
Weight
15328 Da
Superfamily
histone H3 family
Alternative Names
Histone H33, H3f3a, H33a H3-3A H3-3B, H3.3A, H3F3, H3F3A H3.3 histone A histone H3.3|H3 histone family member 3A|H3 histone, family 3A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H3-3a , check out the H3-3a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H3-3a : database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-H3f3b Antibody (C-Term) (M06819-30)
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