Anti-H2AX pS139 H2AFX Antibody

Product Name

Anti-H2AX pS139 H2AFX Antibody

View all H2AX Antibodies

SKU/Catalog Number

A00241

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-H2AX pS139 H2AFX Antibody (Catalog # A00241). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store Histone H2A.X (phospho S139) antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-H2AX pS139 H2AFX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00241)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-H2AX pS139 purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the C-terminal region containing serine 139 of human H2AX protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00241 is reactive to H2AX in Human

Applications

A00241 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

15145 MW

Background of H2AX

Histone H2A.X (phospho S139) antibody is ideal for western blotting and ELISA. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA is wrapped around histone-groups, consisting of the core histones H2A, H2B, H3 and H4. As a reaction on DNA Double-strand breaks (DSB) H2AX becomes phosphorylated on serine 139, called gamma-H2AX. ATM, ATR and PRKDCs, kinases of the PI3-family, are responsible for this phosphorylation. The modification can happen accidentally during replication fork collapse, exogenous genotoxic agents, may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes, in the response to ionizing radiation but also during controlled physiological processes such as V(D)J recombination. Mutagenesis experiments have shown that the modification is necessary for the proper formation of ionizing radiation induced foci in response to double strand breaks, but is not required for the recruitment of proteins to the site of DSBs. Gamma-H2AX is a sensitive target for looking at DSBs in cells. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. The role of the phosphorylated form of the histone in DNA repair is under. Anti-H2AX pS139 is ideal for researched interested in Histones, DNA Damage and Repair, and Epigenetics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000
WB: 1:1,000

Validation Images & Assay Conditions

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Western blot analysis of H2AXpS139 expression in HeLa Lysate stimulated with adriamycin (24 hr) (lane 1) and HeLa Lysate unstimulated (lane 2) using rabbit anti-H2AXpS139 affinity purified polyclonal antibody (Catalog # A00241) at 1:1000. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band(s): none. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For H2AX (Source: Uniprot.org, NCBI)

Alternative Names

H2A histone family, member X; H2A.X; H2a/x; H2AX histone; H2AXhistone H2A.x; YH2AX H2AX H2A.X, H2A/X, H2AFX H2A.X variant histone histone H2AX|H2A histone family member X|H2AX histone|histone H2A.x

For more info on H2AX, check out the H2AX Infographic

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In this infographic, you will see the following information for H2AX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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