Anti-GRAP2 Purified Monoclonal Antibody

Product Name

Anti-GRAP2 Purified Monoclonal Antibody

View all GRAP2 Antibodies

SKU/Catalog Number

M04115

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GRAP2 Purified Monoclonal Antibody (Catalog# M04115). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-GRAP2 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04115)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

UW40

Isotype

Mouse IgG2a

Immunogen

GST-fusion human GRAP2/GADS protein. The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M04115 is reactive to GRAP2 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

37909 MW

Background of GRAP2

GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04115 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 2-6 μg/ml. Intracellular staining.

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For GRAP2 (Source: Uniprot.org, NCBI)

Alternative Names

GADS, GRID, GRAP-2, GrbX GRAP2 GADS, GRAP-2, GRB2L, GRBLG, GRID, GRPL, GrbX, Grf40, Mona, P38 GRB2 related adaptor protein 2 GRB2-related adapter protein 2|GRB-2-like protein|GRB2-related protein with insert domain|SH3-SH2-SH3 adapter Mona|SH3-SH2-SH3 adaptor molecule|adapter protein GRID|grf-40|grf40 adapter protein|growth factor receptor-binding protein|growth factor receptor-bound protein 2-related adaptor protein 2|hematopoietic cell-associated adapter protein GrpL|hematopoietic cell-associated adaptor protein GRPL

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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