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Product Info Summary
| SKU: | A08559 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-GRAH GZMH Antibody
View all Granzyme H Antibodies
SKU/Catalog Number
A08559
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GRAH GZMH Antibody (Catalog # A08559). Tested in WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRAH GZMH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08559)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human GRAH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08559 is reactive to GZMH in Human
Reconstitution
Calculated molecular weight
27315 MW
Background of Granzyme H
Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.
Meier M., Biochemistry 29:4042-4049(1990).
Haddad P., Int. Immunol. 3:57-66(1991).
Klein J.L., Tissue Antigens 35:220-228(1990).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08559 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using GRAH antibody A08559.
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Immunofluorescence analysis of A549 cells, using GRAH antibody A08559.
Protein Target Info & Infographic
Gene/Protein Information For GZMH (Source: Uniprot.org, NCBI)
Gene Name
GZMH
Full Name
Granzyme H
Weight
27315 MW
Superfamily
peptidase S1 family
Alternative Names
Granzyme H;3.4.21.-;CCP-X;Cathepsin G-like 2;CTSGL2;Cytotoxic T-lymphocyte proteinase;Cytotoxic serine protease C;CSP-C;GZMH;CGL2, CTSGL2; GZMH CCP-X, CGL-2, CSP-C, CTLA1, CTSGL2 granzyme H granzyme H|cathepsin G-like 2, protein h-CCPX|cytotoxic T-lymphocyte proteinase|cytotoxic T-lymphocyte-associated serine esterase 1|cytotoxin serine protease-C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GZMH, check out the GZMH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GZMH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRAH GZMH Antibody (A08559)
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No publications found for A08559
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Customer Q&As
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4 Customer Q&As for Anti-GRAH GZMH Antibody
Question
Is a blocking peptide available for product anti-GRAH antibody (A08559)?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We do provide the blocking peptide for product anti-GRAH antibody (A08559). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-GRAH antibody A08559 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
The anti-GRAH antibody (A08559) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-01
Question
Can you help my question with product A08559, anti-GRAH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08559 anti-GRAH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-19
Question
Is this A08559 anti-GRAH antibody reactive to the isotypes of GZMH?
O. Dhar
Verified customer
Asked: 2014-07-10
Answer
The immunogen of A08559 anti-GRAH antibody is Synthesized peptide derived from internal of human GRAH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-07-10
