Click image to see more details
Product Info Summary
| SKU: | M02059 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GPX4 Rabbit Monoclonal Antibody
View all Glutathione Peroxidase 4/GPX4 Antibodies
SKU/Catalog Number
M02059
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GPX4 Rabbit Monoclonal Antibody catalog # M02059. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPX4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02059)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACCO-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GPX4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02059 is reactive to GPX4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
19 kDa
Calculated molecular weight
10180 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02059 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human HepG2 whole cell, human CACO-2 whole cell, human 293T whole cell, human K562 whole cell, rat kidney tissue, rat testis tissue, mouse kidney tissue, mouse testis tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GPX4 using anti-GPX4 antibody (M02059).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX4 antigen affinity purified monoclonal antibody (Catalog # M02059) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 22 kDa.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For GPX4 (Source: Uniprot.org, NCBI)
Gene Name
GPX4
Full Name
Phospholipid hydroperoxide glutathione peroxidase
Weight
10180 MW
Superfamily
glutathione peroxidase family
Alternative Names
Protein S100-A6;Calcyclin;Growth factor-inducible protein 2A9;MLN 4;Prolactin receptor-associated protein;PRA;S100 calcium-binding protein A6;S100A6;CACY; GPX4 GPx-4, GSHPx-4, MCSP, PHGPx, SMDS, snGPx, snPHGPx glutathione peroxidase 4 phospholipid hydroperoxide glutathione peroxidase|epididymis secretory sperm binding protein|phospholipid hydroperoxidase|phospholipid hydroperoxide glutathione peroxidase, mitochondrial|selenoprotein GPX4|sperm nucleus glutathione peroxidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPX4, check out the GPX4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPX4 Rabbit Monoclonal Antibody (M02059)
Hello CJ!
M02059 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhong B,Yu J,Hou Y,Ai N,Ge W,Lu JJ,Chen X.A novel strategy for glioblastoma treatment by induction of noptosis, an NQO1-dependent necrosis.Free Radic Biol Med. 2021 Feb 15:S0891-5849(21)00094-0.doi:10.1016/j.freeradbiomed.2021.02.014.Epub ahead of print.P
Species: Human,Zebrafish
M02059 usage in article: APP:WB, SAMPLE:U251 CELL,U87 CELL,MDA-MB-231 CELL,MCF-7 CELL AND HEK 293T CELL, DILUTION:NA
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Customer Q&As
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16 Customer Q&As for Anti-GPX4 Rabbit Monoclonal Antibody
Question
Our lab were satisfied with the WB result of your anti-GPX4 Rabbit Monoclonal antibody. However we have seen positive staining in right testis isoform cytoplasmic: cytoplasm using this antibody. Is that expected? Could you tell me where is GPX4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
From literature, right testis does express GPX4. Generally GPX4 expresses in isoform mitochondrial: mitochondrion, isoform cytoplasmic: cytoplasm. Regarding which tissues have GPX4 expression, here are a few articles citing expression in various tissues:
Brain, Eye, Lung, Pancreas, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 8039723
Boster Scientific Support
Answered: 2020-02-28
Question
We are currently using anti-GPX4 Rabbit Monoclonal antibody M02059 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-GPX4 Rabbit Monoclonal antibody (M02059) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
We ordered your anti-GPX4 Rabbit Monoclonal antibody for WB on right testis a few years ago. I am using mouse, and We intend to use the antibody for IF next. I am interested in examining right testis as well as pancreas testis in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
I have checked the website and datasheets of our anti-GPX4 Rabbit Monoclonal antibody and I see that M02059 has been validated on mouse in both WB and IF. Thus M02059 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-27
Question
Will anti-GPX4 Rabbit Monoclonal antibody M02059 work on pig WB with liver?
Z. Collins
Verified customer
Asked: 2019-09-02
Answer
Our lab technicians have not validated anti-GPX4 Rabbit Monoclonal antibody M02059 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GPX4 Rabbit Monoclonal antibody M02059 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-02
Question
See below the WB image, lot number and protocol we used for eye using anti-GPX4 Rabbit Monoclonal antibody M02059. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-12
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-GPX4 Rabbit Monoclonal antibody M02059. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
I was wanting to use your anti-GPX4 Rabbit Monoclonal antibody for IHC for mouse eye on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse eye identification?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
As indicated on the product datasheet, M02059 anti-GPX4 Rabbit Monoclonal antibody has been validated for IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-06
Question
I am interested in to test anti-GPX4 Rabbit Monoclonal antibody M02059 on mouse eye for research purposes, then I may be interested in using anti-GPX4 Rabbit Monoclonal antibody M02059 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
The products we sell, including anti-GPX4 Rabbit Monoclonal antibody M02059, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-04
Question
We have observed staining in human lung. Are there any suggestions? Is anti-GPX4 Rabbit Monoclonal antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
From what I have seen in literature lung does express GPX4. From what I have seen in Uniprot.org, GPX4 is expressed in right testis, testis, brain, eye, lung, pancreas testis, liver, among other tissues. Regarding which tissues have GPX4 expression, here are a few articles citing expression in various tissues:
Brain, Eye, Lung, Pancreas, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 8039723
Boster Scientific Support
Answered: 2018-11-28
Question
I have a question about product M02059, anti-GPX4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Edwards
Verified customer
Asked: 2018-11-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02059 anti-GPX4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-28
Question
I see that the anti-GPX4 Rabbit Monoclonal antibody M02059 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-20
Question
Do you have a BSA free version of anti-GPX4 Rabbit Monoclonal antibody M02059 available?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPX4 Rabbit Monoclonal antibody M02059 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-20
Question
Would M02059 anti-GPX4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Brown
Verified customer
Asked: 2017-08-02
Answer
You can see on the product datasheet, M02059 anti-GPX4 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-02
Question
Is a blocking peptide available for product anti-GPX4 Rabbit Monoclonal antibody (M02059)?
B. Bhatt
Verified customer
Asked: 2016-09-20
Answer
We do provide the blocking peptide for product anti-GPX4 Rabbit Monoclonal antibody (M02059). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-09-20
Question
Is this M02059 anti-GPX4 Rabbit Monoclonal antibody reactive to the isotypes of GPX4?
B. Roberts
Verified customer
Asked: 2014-07-18
Answer
The immunogen of M02059 anti-GPX4 Rabbit Monoclonal antibody is A synthesized peptide derived from human GPX4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-07-18
Question
Would anti-GPX4 Rabbit Monoclonal antibody M02059 work for IHC with eye?
J. Singh
Verified customer
Asked: 2013-09-27
Answer
According to the expression profile of eye, GPX4 is highly expressed in eye. So, it is likely that anti-GPX4 Rabbit Monoclonal antibody M02059 will work for IHC with eye.
Boster Scientific Support
Answered: 2013-09-27
