Anti-GPRC5B Antibody

GPRC5B antibody

Boster Bio Anti-GPRC5B Antibody (Catalog# A30826). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Product Info Summary

SKU: A30826
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC, WB

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Product Name

Anti-GPRC5B Antibody

View all GPRC5B Antibodies

SKU/Catalog Number

A30826

Size

100ul

Form

Liquid

Description

Boster Bio Anti-GPRC5B Antibody (Catalog# A30826). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPRC5B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30826)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human GPRC5B. Immunogen sequence location: 61 - 110

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30826 is reactive to GPRC5B in Human, Mouse, Rat

Applications

A30826 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

44795 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For GPRC5B (Source: Uniprot.org, NCBI)

Gene Name

GPRC5B

Full Name

G-protein coupled receptor family C group 5 member B

Weight

44795 MW

Superfamily

G-protein coupled receptor 3 family

Alternative Names

G protein-coupled receptor, family C, group 1, member B; G protein-coupled receptor, family C, group 5, member B; GPRC5B; RAIG2; RAIG-2A-69G12.1; RAIG2G-protein coupled receptor family C group 5 member B; retinoic acid responsive gene protein; Retinoic acid-induced gene 2 protein GPRC5B RAIG-2, RAIG2 G protein-coupled receptor class C group 5 member B G-protein coupled receptor family C group 5 member B|G protein-coupled receptor, family C, group 1, member B|retinoic acid responsive gene protein|retinoic acid-induced gene 2 protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPRC5B, check out the GPRC5B Infographic

GPRC5B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPRC5B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-GPRC5B Antibody

Question

We are currently using anti-GPRC5B antibody A30826 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

The anti-GPRC5B antibody (A30826) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-24

Question

Can you help my question with product A30826, anti-GPRC5B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30826 anti-GPRC5B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-12

Question

Does anti-GPRC5B antibody A30826 work on bovine WB with testis?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

Our lab technicians have not validated anti-GPRC5B antibody A30826 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-GPRC5B antibody A30826 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-26

Order DetailsPrice
A30826

100 μL

$335

Free Secondary Antibody

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A30826
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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