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Product Info Summary
| SKU: | A06269 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GPR35 Antibody
SKU/Catalog Number
A06269
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-GPR35 Antibody (Catalog# A06269). Tested in WB application(s). This antibody reacts with Mouse, Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-GPR35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06269)
Host
Rabbit
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A partial length recombinant GPR35 protein (amino acids 173-305)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06269 is reactive to GPR35 in Human, Mouse
Reconstitution
Calculated molecular weight
34072 MW
Background of GPR35
SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06269 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml
Validation Images & Assay Conditions
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Western blot analysis of GPR35. Anti-GPR35 antibody was used at 2 μg/ml on HCT-116 lysate.
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Western blot analysis of GPR35. Anti-GPR35 antibody was used at 1
Protein Target Info & Infographic
Gene/Protein Information For GPR35 (Source: Uniprot.org, NCBI)
Gene Name
GPR35
Full Name
G-protein coupled receptor 35
Weight
34072 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
G-protein coupled receptor 35;Kynurenic acid receptor;KYNA receptor;GPR35; GPR35 G protein-coupled receptor 35 G-protein coupled receptor 35|KYNA receptor|kynurenic acid receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPR35, check out the GPR35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPR35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPR35 Antibody (A06269)
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No publications found for A06269
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Customer Q&As
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5 Customer Q&As for Anti-GPR35 Antibody
Question
We are currently using anti-GPR35 antibody A06269 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The anti-GPR35 antibody (A06269) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
Does anti-GPR35 antibody A06269 work on feline WB with brain?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
Our lab technicians have not validated anti-GPR35 antibody A06269 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GPR35 antibody A06269 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-24
Question
Will A06269 anti-GPR35 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
It shows on the product datasheet, A06269 anti-GPR35 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-06
Question
Our lab want to know about to test anti-GPR35 antibody A06269 on human brain for research purposes, then I may be interested in using anti-GPR35 antibody A06269 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
The products we sell, including anti-GPR35 antibody A06269, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-06
Question
I was wanting to use your anti-GPR35 antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
A. Singh
Verified customer
Asked: 2017-08-24
Answer
As indicated on the product datasheet, A06269 anti-GPR35 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-24
