Product Info Summary
SKU: | A05432 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-GPAT1 GPAM Antibody
SKU/Catalog Number
A05432
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GPAT1 GPAM Antibody (Catalog # A05432). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
GPAT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GPAT1 GPAM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05432)
Host
Rabbit
Contents
GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1. The immunogen is located within amino acids 730 - 780 of GPAT1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.
Reactive Species
A05432 is reactive to GPAM in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
93795 MW
Background of Gpam
Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05432 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μg/mL.
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Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 μg/mL.
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Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 μg/ml.
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Immunofluorescence of GPAT1 in mouse brain tissue with GPAT1 antibody at 20 μg/mL.
Green: GPAT1 Antibody (A05432)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For GPAM (Source: Uniprot.org, NCBI)
Gene Name
GPAM
Full Name
Glycerol-3-phosphate acyltransferase 1, mitochondrial
Weight
93795 MW
Superfamily
GPAT/DAPAT family
Alternative Names
GPAT, GPAT1, KIAA1560, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT-1 Gpam|GPA, GPAT, GPAT-1, GPAT1, P90|glycerol-3-phosphate acyltransferase, mitochondrial|glycerol-3-phosphate acyltransferase 1, mitochondrial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPAM, check out the GPAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPAT1 GPAM Antibody (A05432)
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