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Product Info Summary
| SKU: | M06975-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-GNG2 Antibody Picoband® (monoclonal, 7C13)
SKU/Catalog Number
M06975-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GNG2 Antibody Picoband® (monoclonal, 7C13) catalog # M06975-1. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GNG2 Antibody Picoband® (monoclonal, 7C13) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06975-1)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
7C13
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human GNG2 recombinant protein (Position: A2-D48).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M06975-1 is reactive to GNG2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
12 kDa
Calculated molecular weight
7.85kDa
Background of Gng2
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 is a protein that in humans is encoded by the GNG2 gene. This gene encodes one of the gamma subunits of a guanine nucleotide-binding protein. Such proteins are involved in signaling mechanisms across membranes. Various subunits forms heterodimers which then interact with the different signal molecules.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06975-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: rat brain tissue, mouse brain tissue
ICC/IF: Caco-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GNG2 using anti-GNG2 antibody (M06975-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNG2 antigen affinity purified monoclonal antibody (Catalog # M06975-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GNG2 at approximately 12 kDa. The expected band size for GNG2 is at 8 kDa.
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Figure 2. IF analysis of GNG2 using anti-GNG2 antibody (M06975-1).
GNG2 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-GNG2 Antibody (M06975-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For GNG2 (Source: Uniprot.org, NCBI)
Gene Name
GNG2
Full Name
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
Weight
7.85kDa
Superfamily
G protein gamma family
Alternative Names
T-complex protein 1 subunit gamma; TCP-1-gamma; CCT-gamma; hTRiC5; CCT3; CCTG; TRIC5 Gng2|guanine nucleotide binding protein (G protein), gamma 2|guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2|g gamma-I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNG2, check out the GNG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GNG2 Antibody Picoband® (monoclonal, 7C13) (M06975-1)
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