Product Info Summary
SKU: | A03643-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-GMPS Antibody Picoband®
SKU/Catalog Number
A03643-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GMPS Antibody Picoband® catalog # A03643-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GMPS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03643-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GMPS recombinant protein (Position: H21-K671).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03643-1 is reactive to GMPS in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
77 kDa
Calculated molecular weight
28461 MW
Background of GMPS
Guanosine monophosphate synthetase, also known as GMPS is an enzyme that converts xanthosine monophosphate to guanosine monophosphate. In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03643-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, Human
Positive Control
WB: human HEK293 whole cell, human A431 whole cell, human HL-60 whole cell, human COLO320 whole cell, human Raji whole cell, human THP-1 whole cell, human Jurkat whole cell, human K562 whole cell, rat PC-12 whole cell, mouse thymus tissue
FCM: HELA cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GMPS using anti-GMPS antibody (A03643-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human COLO320 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human THP-1 whole cell lysates,
Lane 7: human Jurkat whole cell lysates,
Lane 8: human K562 whole cell lysates,
Lane 9: rat PC-12 whole cell lysates,
Lane 10: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GMPS antigen affinity purified polyclonal antibody (Catalog # A03643-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GMPS at approximately 77KD. The expected band size for GMPS is at 77KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of HELA cells using anti-GMPS antibody (A03643-1).
Overlay histogram showing HELA cells stained with A03643-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GMPS Antibody (A03643-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GMPS (Source: Uniprot.org, NCBI)
Gene Name
GMPS
Full Name
GMP synthase [glutamine-hydrolyzing]
Weight
28461 MW
Alternative Names
DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46; MCM5; CDC46 GMPS GATD7 guanine monophosphate synthase GMP synthase [glutamine-hydrolyzing]|GMP synthase|GMP synthetase|MLL/GMPS fusion protein|glutamine amidotransferase|guanine monophosphate synthetase|guanosine 5-monophosphate synthase|testicular tissue protein Li 82
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GMPS, check out the GMPS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GMPS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GMPS Antibody Picoband® (A03643-1)
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