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Product Info Summary
| SKU: | PB9003 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GM-CSF/CSF2 Antibody Picoband®
SKU/Catalog Number
PB9003
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GM-CSF/CSF2 Antibody Picoband® catalog # PB9003. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GM-CSF/CSF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9003)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9003 is reactive to Csf2 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
16091 MW
Background of GM-CSF
GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9003 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
IHC: mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of GM-CSF using anti-GM-CSF antibody (PB9003).
GM-CSF was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GM-CSF Antibody (PB9003) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Western blot analysis of GM-CSF using anti-GM-CSF antibody (PB9003).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse GM-CSF protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GM-CSF antigen affinity purified polyclonal antibody (Catalog # PB9003) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GM-CSF at approximately 17 kDa. The expected band size for GM-CSF is at 17 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Csf2 (Source: Uniprot.org, NCBI)
Gene Name
Csf2
Full Name
Granulocyte-macrophage colony-stimulating factor
Weight
16091 MW
Superfamily
GM-CSF family
Alternative Names
Granulocyte-macrophage colony-stimulating factor;GM-CSF;Colony-stimulating factor;CSF;Csf2;Csfgm; CSF2 CSF, GMCSF colony stimulating factor 2 granulocyte-macrophage colony-stimulating factor|colony stimulating factor 2 (granulocyte-macrophage)|granulocyte macrophage-colony stimulating factor|molgramostim|molgramostin|sargramostim
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Csf2, check out the Csf2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Csf2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GM-CSF/CSF2 Antibody Picoband® (PB9003)
Hello CJ!
PB9003 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Balance between Conventional DCs and Plasmacytoid DCs Is Pivotal for Immunological Tolerance during Pregnancy in the Mouse
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Customer Q&As
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17 Customer Q&As for Anti-GM-CSF/CSF2 Antibody Picoband®
Question
Would anti-GM-CSF/CSF2 antibody PB9003 work on pig WB with peripheral blood?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
Our lab technicians have not tested anti-GM-CSF/CSF2 antibody PB9003 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GM-CSF/CSF2 antibody PB9003 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig peripheral blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-17
Question
Is this PB9003 anti-GM-CSF/CSF2 antibody reactive to the isotypes of CSF2?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The immunogen of PB9003 anti-GM-CSF/CSF2 antibody is E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-25
Question
Do you have a BSA free version of anti-GM-CSF/CSF2 antibody PB9003 available?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GM-CSF/CSF2 antibody PB9003 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for upper lobe of left lung using anti-GM-CSF/CSF2 antibody PB9003. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
We have observed staining in mouse upper lobe of left lung. Are there any suggestions? Is anti-GM-CSF/CSF2 antibody supposed to stain upper lobe of left lung positively?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Based on literature upper lobe of left lung does express CSF2. Based on Uniprot.org, CSF2 is expressed in upper lobe of left lung, peripheral blood, among other tissues. Regarding which tissues have CSF2 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 1737041
Boster Scientific Support
Answered: 2019-09-11
Question
Our lab were satisfied with the WB result of your anti-GM-CSF/CSF2 antibody. However we have been able to see positive staining in peripheral blood secreted using this antibody. Is that expected? Could you tell me where is CSF2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
From what I have seen in literature, peripheral blood does express CSF2. Generally CSF2 expresses in secreted. Regarding which tissues have CSF2 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 1737041
Boster Scientific Support
Answered: 2019-09-05
Question
I was wanting to use your anti-GM-CSF/CSF2 antibody for WB for mouse upper lobe of left lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse upper lobe of left lung identification?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
It shows on the product datasheet, PB9003 anti-GM-CSF/CSF2 antibody has been validated for IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse upper lobe of left lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-21
Question
I see that the anti-GM-CSF/CSF2 antibody PB9003 works with WB, what is the protocol used to produce the result images on the product page?
C. Wu
Verified customer
Asked: 2019-06-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-20
Question
Will anti-GM-CSF/CSF2 antibody PB9003 work for WB with upper lobe of left lung?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
According to the expression profile of upper lobe of left lung, CSF2 is highly expressed in upper lobe of left lung. So, it is likely that anti-GM-CSF/CSF2 antibody PB9003 will work for WB with upper lobe of left lung.
Boster Scientific Support
Answered: 2019-06-07
Question
We are currently using anti-GM-CSF/CSF2 antibody PB9003 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on monkey tissues as well?
L. Johnson
Verified customer
Asked: 2018-11-22
Answer
The anti-GM-CSF/CSF2 antibody (PB9003) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-22
Question
Does PB9003 anti-GM-CSF/CSF2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Roberts
Verified customer
Asked: 2018-04-17
Answer
You can see on the product datasheet, PB9003 anti-GM-CSF/CSF2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-17
Question
We have tried in the past anti-GM-CSF/CSF2 antibody for IHC on peripheral blood in the past. I am using mouse, and We intend to use the antibody for WB next. We need examining peripheral blood as well as upper lobe of left lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
J. Evans
Verified customer
Asked: 2018-02-01
Answer
I have checked the website and datasheets of our anti-GM-CSF/CSF2 antibody and it seems that PB9003 has been validated on mouse in both IHC and WB. Thus PB9003 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-02-01
Question
I have a question about product PB9003, anti-GM-CSF/CSF2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9003 anti-GM-CSF/CSF2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-11
Question
Our lab want to know about using your anti-GM-CSF/CSF2 antibody for monocyte differentiation studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
We appreciate your inquiry. This PB9003 anti-GM-CSF/CSF2 antibody is tested on mouse spleen tissue. It is guaranteed to work for IHC, WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-11-24
Question
Is a blocking peptide available for product anti-GM-CSF/CSF2 antibody (PB9003)?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
We do provide the blocking peptide for product anti-GM-CSF/CSF2 antibody (PB9003). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-14
Question
Here is the WB image, lot number and protocol we used for upper lobe of left lung using anti-GM-CSF/CSF2 antibody PB9003. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-08
Question
My question regards to test anti-GM-CSF/CSF2 antibody PB9003 on mouse upper lobe of left lung for research purposes, then I may be interested in using anti-GM-CSF/CSF2 antibody PB9003 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Thomas
Verified customer
Asked: 2016-05-18
Answer
The products we sell, including anti-GM-CSF/CSF2 antibody PB9003, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-18
