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Product Info Summary
| SKU: | A03871 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Glypican-1 Antibody
View all Glypican 1 Antibodies
SKU/Catalog Number
A03871
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Glypican-1 Antibody (Catalog # A03871). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Glypican-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03871)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Glypican-1 protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03871 is reactive to GPC1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
52456 MW
Background of Glypican 1
Glypican-1 antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03871 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: 10µg/mL
WB: 1:500- 1:2,000
Validation Images & Assay Conditions
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Figure 1. IHC analysis of GPC1 using anti-GPC1 antibody (A03871).
GPC1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GPC1 Antibody (A03871) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. Western blot analysis of GPC1 using anti-GPC1 antibody (A03871).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPC1 antigen affinity purified polyclonal antibody (Catalog # A03871) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GPC1.
Protein Target Info & Infographic
Gene/Protein Information For GPC1 (Source: Uniprot.org, NCBI)
Gene Name
GPC1
Full Name
Glypican-1
Weight
52456 MW
Superfamily
glypican family
Alternative Names
Glypican-1, Glypican 1, Glypican1, Secreted glypican-1, GPC1, FLJ38078 GPC1 glypican glypican 1 glypican-1|epididymis secretory sperm binding protein|glypican proteoglycan 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPC1, check out the GPC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glypican-1 Antibody (A03871)
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Customer Q&As
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5 Customer Q&As for Anti-Glypican-1 Antibody
Question
We want to test anti-Glypican-1 antibody A03871 on rat heart left ventricle for research purposes, then I may be interested in using anti-Glypican-1 antibody A03871 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
The products we sell, including anti-Glypican-1 antibody A03871, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-01
Question
Is there a BSA free version of anti-Glypican-1 antibody A03871 available?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Glypican-1 antibody A03871 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-16
Question
We are currently using anti-Glypican-1 antibody A03871 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
P. Mitchell
Verified customer
Asked: 2016-05-16
Answer
The anti-Glypican-1 antibody (A03871) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-16
Question
Does anti-Glypican-1 antibody A03871 work for WB with heart left ventricle?
J. Jones
Verified customer
Asked: 2015-05-08
Answer
According to the expression profile of heart left ventricle, GPC1 is highly expressed in heart left ventricle. So, it is likely that anti-Glypican-1 antibody A03871 will work for WB with heart left ventricle.
Boster Scientific Support
Answered: 2015-05-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-Glypican-1 antibody A03871. Let me know if you need anything else.
C. Zhao
Verified customer
Asked: 2014-10-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-21
