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Product Info Summary
| SKU: | A00877 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Homeobox protein CDX-2 Cdx2 Antibody
SKU/Catalog Number
A00877
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Homeobox protein CDX-2 Cdx2 Antibody (Catalog # A00877). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein CDX-2 Cdx2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00877)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.12~16(S-M-Y-P-S) derived from Human Cdx2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00877 is reactive to CDX2 in Human, Monkey, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
74605 MW
Background of CDX2
Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Inamori K., Takeshita K., Chiba S., Yazaki Y., Hirai H.Biochem. Biophys. Res. Commun. 196:203-208(1993)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00877 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 40kd
Western blotting: 1:500~1:1000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CDX2 using anti-CDX2 antibody (A00877).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDX2 antigen affinity purified polyclonal antibody (Catalog # A00877) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CDX2.
Protein Target Info & Infographic
Gene/Protein Information For CDX2 (Source: Uniprot.org, NCBI)
Gene Name
CDX2
Full Name
Homeobox protein CDX-2
Weight
74605 MW
Superfamily
Caudal homeobox family
Alternative Names
Delta-like protein 4;Drosophila Delta homolog 4;Delta4;DLL4;UNQ1895/PRO4341; CDX2 CDX-3/AS, CDX3, CDX2 caudal type homeobox 2 homeobox protein CDX-2|caudal type homeobox transcription factor 2|caudal-type homeobox protein 2|homeobox protein miniCDX2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDX2, check out the CDX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein CDX-2 Cdx2 Antibody (A00877)
Hello CJ!
A00877 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Overexpression of caudal-related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice
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