Anti-Homeobox protein CDX-2 Cdx2 Antibody

Product Name

Anti-Homeobox protein CDX-2 Cdx2 Antibody

View all CDX2 Antibodies

SKU/Catalog Number

A00877

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Homeobox protein CDX-2 Cdx2 Antibody (Catalog # A00877). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Homeobox protein CDX-2 Cdx2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00877)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa.12~16(S-M-Y-P-S) derived from Human Cdx2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00877 is reactive to CDX2 in Human, Monkey, Mouse, Rat

Applications

A00877 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

74605 MW

Background of CDX2

Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

Inamori K., Takeshita K., Chiba S., Yazaki Y., Hirai H.Biochem. Biophys. Res. Commun. 196:203-208(1993)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 40kd
Western blotting: 1:500~1:1000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CDX2 using anti-CDX2 antibody (A00877).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDX2 antigen affinity purified polyclonal antibody (Catalog # A00877) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CDX2.

Gene/Protein Information For CDX2 (Source: Uniprot.org, NCBI)

Alternative Names

caudal type homeo box transcription factor 2; caudal type homeobox 2; Caudal-type homeobox protein 2; CDX2; CDX3; CDX-3; CDX3caudal type homeobox transcription factor 2; homeobox protein CDX-2 CDX2 CDX-3/AS, CDX3, CDX2 caudal type homeobox 2 homeobox protein CDX-2|caudal type homeobox transcription factor 2|caudal-type homeobox protein 2|homeobox protein miniCDX2

For more info on CDX2, check out the CDX2 Infographic

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In this infographic, you will see the following information for CDX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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