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Product Info Summary
| SKU: | A03392-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Glutaredoxin 1/GLRX Antibody Picoband®
View all Glutaredoxin 1/GLRX1 Antibodies
SKU/Catalog Number
A03392-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glutaredoxin 1/GLRX Antibody Picoband® catalog # A03392-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Glutaredoxin 1/GLRX Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03392-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Glutaredoxin 1/GLRX recombinant protein (Position: M1-Q106).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03392-1 is reactive to GLRX in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
12 kDa
Calculated molecular weight
11.776kDa
Background of Glutaredoxin 1/GLRX1
Glutaredoxin-1 is a protein that in humans is encoded by the GLRX gene. This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03392-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human U-87MG whole cell, human PC-3 whole cell
ICC/IF: A431 cell
FCM: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Glutaredoxin 1/GLRX using anti-Glutaredoxin 1/GLRX antibody (A03392-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutaredoxin 1/GLRX antigen affinity purified polyclonal antibody (Catalog # A03392-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutaredoxin 1/GLRX at approximately 12 kDa. The expected band size for Glutaredoxin 1/GLRX is at 12 kDa.
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Figure 2. IF analysis of Glutaredoxin 1/GLRX using anti-Glutaredoxin 1/GLRX antibody (A03392-1).
Glutaredoxin 1/GLRX was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Glutaredoxin 1/GLRX Antibody (A03392-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of Hela cells using anti-Glutaredoxin 1/GLRX antibody (A03392-1).
Overlay histogram showing Hela cells stained with A03392-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutaredoxin 1/GLRX Antibody (A03392-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GLRX (Source: Uniprot.org, NCBI)
Gene Name
GLRX
Full Name
Glutaredoxin-1
Weight
11.776kDa
Superfamily
glutaredoxin family
Alternative Names
Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component; Gc GLRX GRX, GRX1 glutaredoxin glutaredoxin-1|TTase-1|thioltransferase|thioltransferase-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLRX, check out the GLRX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLRX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glutaredoxin 1/GLRX Antibody Picoband® (A03392-1)
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