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Product Info Summary
| SKU: | A06552 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GluR4 GRM4 Antibody
SKU/Catalog Number
A06552
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GluR4 GRM4 Antibody (Catalog # A06552). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GluR4 GRM4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06552)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human GluR4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06552 is reactive to GRM4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
101868 MW
Background of mGluR4
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
Susana Santos Correia, J. Biol. Chem., Feb 2003; 278: 6307 - 6313.
Maxim Mokin, J Neurophysiol, Jan 2006; 95: 215 - 224.
Maxim Mokin, J Neurophysiol, Jan 2006; 95: 215 - 224.
Peyman Golshani, PNAS, Mar 2001; 98: 4172.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06552 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from JK cells (Lane 2), using mGluR4 antiobdy A06552. The lane on the left is treated with synthesized peptide.
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Western blot analysis of extracts from 293 cells, untreated or treated with Forskolin (40nM, 30mins), using GluR4 antibody A06552.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using GluR4 antibody A06552.
Protein Target Info & Infographic
Gene/Protein Information For GRM4 (Source: Uniprot.org, NCBI)
Gene Name
GRM4
Full Name
Metabotropic glutamate receptor 4
Weight
101868 MW
Superfamily
G-protein coupled receptor 3 family
Alternative Names
Metabotropic glutamate receptor 4;mGluR4;GRM4;GPRC1D, MGLUR4; GRM4 GPRC1D, MGLUR4, mGlu4 glutamate metabotropic receptor 4 metabotropic glutamate receptor 4|glutamate receptor, metabotropic 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRM4, check out the GRM4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GluR4 GRM4 Antibody (A06552)
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Customer Q&As
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5 Customer Q&As for Anti-GluR4 GRM4 Antibody
Question
Is there a BSA free version of anti-GluR4 antibody A06552 available?
B. Baker
Verified customer
Asked: 2020-01-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GluR4 antibody A06552 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-24
Question
We are currently using anti-GluR4 antibody A06552 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The anti-GluR4 antibody (A06552) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-28
Question
Does A06552 anti-GluR4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
As indicated on the product datasheet, A06552 anti-GluR4 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-14
Question
See attached the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti-GluR4 antibody A06552. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-05
Question
I was wanting to use your anti-GluR4 antibody for IHC for mouse right hemisphere of cerebellum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right hemisphere of cerebellum identification?
S. Edwards
Verified customer
Asked: 2015-09-14
Answer
As indicated on the product datasheet, A06552 anti-GluR4 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse right hemisphere of cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-14
