Product Info Summary
SKU: | M06114 |
---|---|
Size: | 100 ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Lipocalin-1 LCN1 Monoclonal Antibody
View all Lipocalin-1 Antibodies
SKU/Catalog Number
M06114
Size
100 ul
Form
Liquid
Description
Boster Bio Anti-Lipocalin-1 LCN1 Monoclonal Antibody catalog # M06114. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lipocalin-1 LCN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06114)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
Clonality
Monoclonal
Clone Number
10B10
Isotype
IgG1
Immunogen
Purified recombinant fragment of Lipocalin-1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06114 is reactive to LCN1 in Human
Applications
M06114 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
19250 MW
Background of Lipocalin-1
Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
![m06114 anti lipocalin 1 lcn1 monoclonal antibody if testing 3 m06114 anti lipocalin 1 lcn1 monoclonal antibody if testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m06114-anti-lipocalin-1-lcn1-monoclonal-antibody-if-testing-3.jpg)
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Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with LCN1-hIgGFc using Lipocalin-1 monoclonal antibody (green) , showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
![m06114 anti lipocalin 1 lcn1 monoclonal antibody ihc testing 2 m06114 anti lipocalin 1 lcn1 monoclonal antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m06114-anti-lipocalin-1-lcn1-monoclonal-antibody-ihc-testing-2.jpg)
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Immunohistochemistry analysis of paraffin-embedded human Prostate tissues with AEC staining using Lipocalin-1 monoclonal antibody.
![m06114 anti lipocalin 1 lcn1 monoclonal antibody wb testing 1 m06114 anti lipocalin 1 lcn1 monoclonal antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m06114-anti-lipocalin-1-lcn1-monoclonal-antibody-wb-testing-1.jpg)
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Western blot analysis using Lipocalin-1 monoclonal antibody against HEK293 (1) and LCN1-hIgGFc transfected HEK293 cell lysate (2).
Protein Target Info & Infographic
Gene/Protein Information For LCN1 (Source: Uniprot.org, NCBI)
Gene Name
LCN1
Full Name
Lipocalin-1
Weight
19250 MW
Superfamily
calycin superfamily
Alternative Names
LCN1; lipocalin 1 (tear prealbumin); lipocalin 1-like 2; Lipocalin1; Lipocalin-1; MGC71975; PMFA; Tear lipocalin; Tear Prealbumin; TLC; TPVon Ebner gland protein; VEG protein; VEGP; VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin) LCN1 PMFA, TLC, TP, VEGP lipocalin 1 lipocalin-1|VEG protein|Von Ebner gland protein|protein migrating faster than albumin|tear prealbumin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LCN1, check out the LCN1 Infographic
![LCN1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LCN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lipocalin-1 LCN1 Monoclonal Antibody (M06114)
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