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Product Info Summary
| SKU: | M00678 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Glucagon GCG Rabbit Monoclonal Antibody
SKU/Catalog Number
M00678
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Glucagon GCG Rabbit Monoclonal Antibody catalog # M00678. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucagon GCG Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00678)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOAF-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Glucagon
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00678 is reactive to GCG in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
149 kDa
Calculated molecular weight
20909 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00678 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: HepG2 cell
IHC: mouse pancrease tissue
Validation Images & Assay Conditions
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Western blot analysis of Glucagon expression in HepG2 cell lysate.
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Figure 2. IHC analysis of Glucagon using anti-Glucagon antibody (M00678).
Glucagon was detected in a paraffin-embedded section of mouse pancrease tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Glucagon Antibody (M00678) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GCG (Source: Uniprot.org, NCBI)
Gene Name
GCG
Full Name
Glucagon
Weight
20909 MW
Superfamily
glucagon family
Alternative Names
Glucagon;Glicentin;Glicentin-related polypeptide;GRPP;Oxyntomodulin;OXM;OXY;Glucagon;Glucagon-like peptide 1;GLP-1;Incretin hormone;Glucagon-like peptide 1 (7-37);GLP-1 (7-37);Glucagon-like peptide 1 (7-36);GLP-1 (7-36);Glucagon-like peptide 2;GLP-2;GCG; GCG GLP-1, GLP1, GLP2, GRPP glucagon pro-glucagon|glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide 2|preproglucagon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCG, check out the GCG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glucagon GCG Rabbit Monoclonal Antibody (M00678)
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