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Product Info Summary
| SKU: | MA1047 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rabbit, Rat |
| Host: | Mouse |
| Application: | IF, IHC |
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Product info
Product Name
Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10)
SKU/Catalog Number
MA1047
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10) catalog # MA1047. Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1047)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
K79bB10
Isotype
Mouse IgG1
Immunogen
Polymerized porcine glucagon.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1047 is reactive to Gcg in Human, Mouse, Rabbit, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
20846 MW
Background of GCG
Glucagon is a member of a multigene family that includes secretin. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. The human glucagon gene is approximately 9.4 kb long and contains 6 exons and 5 introns, and assigned to 2q36-2q37.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1047 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat
Immunofluorescence, 5 μg/ml, Human
Positive Control
IHC: Rat Pancreas tissue
IF: mouse pancreas tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Glucagon using anti-Glucagon antibody (MA1047).
Glucagon was detected in a paraffin-embedded section of Rat Pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 2. IF analysis of Glucagon using anti-Glucagon antibody (MA1047).
Glucagon was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Gcg (Source: Uniprot.org, NCBI)
Gene Name
Gcg
Full Name
Glucagon
Weight
20846 MW
Superfamily
glucagon family
Alternative Names
Glucagon;Glicentin;Glicentin-related polypeptide;GRPP;Oxyntomodulin;OXM;OXY;Glucagon;Glucagon-like peptide 1;GLP-1;Glucagon-like peptide 1 (7-37);GLP-1 (7-37);Glucagon-like peptide 1 (7-36);GLP-1 (7-36);Glucagon-like peptide 2;GLP-2;Gcg; GCG GLP-1, GLP1, GLP2, GRPP glucagon pro-glucagon|glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide 2|preproglucagon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Gcg, check out the Gcg Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Gcg: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10) (MA1047)
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Customer Q&As
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5 Customer Q&As for Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10)
Question
Is this MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) reactive to the isotypes of GCG?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
The immunogen of MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) is Polymerized porcine glucagon. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-04
Question
Can you help my question with product MA1047, anti-Glucagon antibody (Monoclonal, K79bB10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1047 anti-Glucagon antibody (Monoclonal, K79bB10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-11
Question
I was wanting to use your anti-Glucagon antibody (Monoclonal, K79bB10) for IHC for rat pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat pancreas identification?
K. Thomas
Verified customer
Asked: 2017-10-09
Answer
It shows on the product datasheet, MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) has been tested for IHC on human, mouse, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-09
Question
Will MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Kulkarni
Verified customer
Asked: 2016-05-16
Answer
As indicated on the product datasheet, MA1047 anti-Glucagon antibody (Monoclonal, K79bB10) as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-05-16
Question
I have attached the WB image, lot number and protocol we used for pancreas using anti-Glucagon antibody (Monoclonal, K79bB10) MA1047. Please let me know if you require anything else.
J. Jones
Verified customer
Asked: 2014-07-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-10
