Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10)

Product Name

Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10)

View all GCG Antibodies

SKU/Catalog Number

MA1047

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10) catalog # MA1047. Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Glucagon Gcg Antibody (Monoclonal, K79bB10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1047)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

K79bB10

Isotype

Mouse IgG1

Immunogen

Polymerized porcine glucagon.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1047 is reactive to GCG in Human, Mouse, Rabbit, Rat

Applications

MA1047 is guaranteed for IF, IHC Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

20846 MW

Background of GCG

Glucagon is a member of a multigene family that includes secretin. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. The human glucagon gene is approximately 9.4 kb long and contains 6 exons and 5 introns, and assigned to 2q36-2q37.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, rabbit, By Heat
Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. IHC analysis of Glucagon using anti-Glucagon antibody (MA1047).
Glucagon was detected in a paraffin-embedded section of Rat Pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

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Figure 2. IF analysis of Glucagon using anti-Glucagon antibody (MA1047).
Glucagon was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For GCG (Source: Uniprot.org, NCBI)

Alternative Names

GCG; glicentin-related polypeptide; GLP1; GLP-1; Glucagon; glucagon-like peptide 1; glucagon-like peptide 2; GRPP GCG GLP-1, GLP1, GLP2, GRPP glucagon pro-glucagon|glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide 2|preproglucagon

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In this infographic, you will see the following information for GCG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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