Anti-Gap junction beta-2 protein GJB2 Antibody

Product Name

Anti-Gap junction beta-2 protein GJB2 Antibody

View all Connexin 26/GJB2 Antibodies

SKU/Catalog Number

PA1025

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Gap junction beta-2 protein GJB2 Antibody catalog # PA1025. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Gap junction beta-2 protein GJB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1025)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GJB2, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1025 is reactive to GJB2 in Human, Mouse, Rat

Applications

PA1025 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

82 kDa

Calculated molecular weight

26215 MW

Background of Connexin 26/GJB2

Connexin26 (CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5% identity with rat Cx26. connexin 26 (GJB2) is assigned to human chromosome 13q11-q12 .Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Click image to see more details

Anti-GJB2 antibody, PA1025, Western blotting
WB: Rat Liver Tissue Lysate

Gene/Protein Information For GJB2 (Source: Uniprot.org, NCBI)

Alternative Names

beta 2, 26kD (connexin 26); Connexin 26; CX26; CX26DFNA3; DFNA3; DFNA3A; DFNB1; gap junction protein, beta 2, 26kDa (connexin 26); gap junction protein, beta 2, 26kDa; GJB2; HID; KID; NSRD1; PPK GJB2 BAPS, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, HID, KID, NSRD1, PPK gap junction protein beta 2 gap junction beta-2 protein|connexin 26|gap junction beta 2 proteinc|gap junction protein, beta 2, 26kDa|mutant gap junction beta 2 protein|mutant gap junction protein beta 2

For more info on GJB2, check out the GJB2 Infographic

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In this infographic, you will see the following information for GJB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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