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Product Info Summary
| SKU: | A03125-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GITR/Tnfrsf18 Picoband® Antibody
View all GITR/TNFRSF18 Antibodies
SKU/Catalog Number
A03125-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GITR/Tnfrsf18 Picoband® Antibody catalog # A03125-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GITR/Tnfrsf18 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03125-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse GITR/Tnfrsf18 recombinant protein (Position: N41-T155).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03125-3 is reactive to Tnfrsf18 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30-35 kDa
Calculated molecular weight
26000 MW
Background of GITR/TNFRSF18
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18) also known as activation-inducible TNFR family receptor (AITR) or glucocorticoid-induced TNFR-related protein (GITR) is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 4; 4 E2. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03125-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat thymus tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tnfrsf18 using anti-Tnfrsf18 antibody (A03125-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tnfrsf18 antigen affinity purified polyclonal antibody (Catalog # A03125-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tnfrsf18 at approximately 30-35KD. The expected band size for Tnfrsf18 is at 26KD.
Protein Target Info & Infographic
Gene/Protein Information For Tnfrsf18 (Source: Uniprot.org, NCBI)
Gene Name
Tnfrsf18
Full Name
Tumor necrosis factor receptor superfamily member 18
Weight
26000 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; CD357; Tnfrsf18; Gitr TNFRSF18 AITR, CD357, ENERGEN, GITR, GITR-D TNF receptor superfamily member 18 tumor necrosis factor receptor superfamily member 18|TNF receptor superfamily activation-inducible protein|activation-inducible TNFR family receptor|glucocorticoid-induced TNFR-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnfrsf18, check out the Tnfrsf18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnfrsf18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GITR/Tnfrsf18 Picoband® Antibody (A03125-3)
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