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Facts about Tumor necrosis factor receptor superfamily member 18.
Receptor for TNFSF18.
Appears to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death.Mediated NF-kappa-B activation through the TRAF2/NIK pathway. .
Human | |
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Gene Name: | TNFRSF18 |
Uniprot: | Q9Y5U5 |
Entrez: | 8784 |
Belongs to: |
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No superfamily |
Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18
Mass (kDA):
26 kDA
Human | |
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Location: | 1p36.33 |
Sequence: | 1; NC_000001.11 (1203508..1207901, complement) |
Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
BosterBio Anti-GITR/Tnfrsf18 Antibody (tm) is available for purchase. This antibody validates TNFRSF18 proteins and expression levels by using SYBR Green PCR Master Mix. We will discuss the validation and applications of this antibody in this article. We will also discuss the benefits of this antibody.
An agonist anti–GITR mAb can increase T-cell response during vaccination, but it could also increase autoimmunity. The antibody increases the risk of infection. Careful selection of vaccine targets is essential, as well as monitoring for immunotoxicity. This antibody can also inhibit tumor growth by increasing T-cell recall responses.
The GITR is a key target in immunotherapy research. Knockout studies in mice have shown that the GITR receptor regulates both CD3 driven T cell activation as well as programmed cell death. The GITR specific mAb DTA-1 inducing GITR signaling is an agonist.
The GITR/TNFRSF18 antigen targets TNFRSSF18. TNFSF18, a member of TNF-receptor Superfamily. It functions by binding to many receptors and is implicated with a variety of biological processes. Although its role in autoimmune disease remains unclear, knockout studies on mice have shown that it may play a part in neuroprotection.
The antibody is believed to enhance CD8 T-cell response to tumors, according to studies. Combining antiGITR mAb with chemotherapeutic medications has been shown to increase antitumor efficacy in mouse models. It has a positive impact on CD8+T cells. It induces a memory-dependent immune response.
Quantitative real time PCR allows researchers to determine the starting nucleic acid concentration. This method has several advantages over traditional quantitative PCR. It is sensitiver and can produce a wider dynamic spectrum of results. It can detect even the smallest changes in gene levels. Third, quantitative real-time PCR has been deemed the gold standard to confirm microarray gene transcription data.
The amplification plot technique is based on a simple algorithm for calculating the relative efficiency of each sample. This information is used to determine the gene expression. The DataAssist(tm) Software from Applied Biosystems is a user-friendly data analysis tool. It offers multiple statistical functions and an interactive visualization tool.
SYBR GreenPCR Master Mix is 2-fold concentrated solution containing dNTPs (dNTPs), MgCl2 and DNA polymerase. Different reagents come with different dyes. SYBR Green PCR Master Mix also includes a passive refer dye to improve the accuracy of the results. This internal reference dye corrects optical variations between wells and normalizes fluorescence signal.
Real-timePCR allows scientists and doctors to compare the expression levels between genes. It is widely used for determining the effect of drug therapy on the body. This method does have its downsides. While it is sensitive, it is expensive and requires specialized equipment and reagents. If you are interested in using quantitative real-time PCR, consider using the SYBR Green PCR Master Mix.
A high sensitivity indicates that it can detect small variations in gene expression in melanoma cellular cells. It requires sound experimental design. Also, a thorough understanding of normalization processes. If you have many samples, the SYBR Green Master Mix can be used as a reference. So get started with your next quantitative realtime PCR experiment.
GITR/TNFRSF18 monoclonal Antibody can detect TNFRSF18 via Western blots as well as ELISAs. It is cross-reactive with human GITR recombinant and mouse 4-1BB. It can be used in sandwich immunoassays. You can access the manufacturer's website for the general protocol.
TNFSF18 receptor is encoded by the TNFRSF18 gene. The protein is involved in interactions between activated T-lymphocytes and endothelial cells and is a potential regulator of T-cell receptor-mediated cell death. It is encoded by three alternative transcript variants, resulting in three isoforms. Other uses of this gene are not yet known.
GITR belongs to the TNF-receptor superfamily. It is expressed at low levels in resting T cell lymphocytes. It is increased upon activation and inhibits the inhibitory function in CD25+CD4+ Tregs cells. Activated T cells have high levels of GITR Ioforms B, and C. They also display splicing in the cytoplasmic tails.
Humans with advanced glioma were used to validate the TNFRSF18 plasma gene expression mark. This marker is also related to a number other proteins, such as the RANK pathway. The markers were found to be significantly associated with VPD. They were also positively correlated with plasma gene expression of OPG and progesterone. TNFRSF18 was found to be negatively correlated with STAT1 and STAT2 gene expressions, while RANK and RANK had negative correlations.
These results indicate that BACE1 and TNFRSF18 expressions were significantly associated with several immune checkpoint gene expressions. BACE1-AS expression was negatively correlated with TNFSF25 expression, while it was positively correlated to LGALS9. The patient's outlook was also affected in part by NRP1 (the marker for BACE1 -AS) and BACE1 -AS (the marker for NRP1). The area under a time-dependent survival curve for one-year survivors and three-year survivors was 0.610 and 0.613 respectively. This suggests that the markers can be powerful tools for cancer diagnosis.
PMID: 10074428 by Gurney A.L., et al. Identification of a new member of the tumor necrosis factor family and its receptor, a human ortholog of mouse GITR.
PMID: 10037686 by Kwon B., et al. Identification of a novel activation-inducible protein of the tumor necrosis factor receptor superfamily and its ligand.