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Product Info Summary
| SKU: | A03161 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GGH/Gamma Glutamyl Hydrolase Antibody
View all gamma-glutamyl hydrolase Antibodies
SKU/Catalog Number
A03161
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GGH/Gamma Glutamyl Hydrolase Antibody (Catalog # A03161). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GGH/Gamma Glutamyl Hydrolase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03161)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human GGH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03161 is reactive to GGH in Human, Mouse
Reconstitution
Calculated molecular weight
35964 MW
Background of gamma-glutamyl hydrolase
Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
Yao R., Proc. Natl. Acad. Sci. U.S.A. 93:10134-10138(1996).
Yin D., Gene 238:463-470(1999).
Basrur V., J. Proteome Res. 2:69-79(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03161 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from RAW264.7 cells, using GGH antibody A03161.
Protein Target Info & Infographic
Gene/Protein Information For GGH (Source: Uniprot.org, NCBI)
Gene Name
GGH
Full Name
Gamma-glutamyl hydrolase
Weight
35964 MW
Superfamily
peptidase C26 family
Alternative Names
Gamma-glutamyl hydrolase;3.4.19.9;Conjugase;GH;Gamma-Glu-X carboxypeptidase;GGH; GGH GH gamma-glutamyl hydrolase gamma-glutamyl hydrolase|gamma-Glu-X carboxypeptidase|gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GGH, check out the GGH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GGH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GGH/Gamma Glutamyl Hydrolase Antibody (A03161)
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Customer Q&As
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7 Customer Q&As for Anti-GGH/Gamma Glutamyl Hydrolase Antibody
Question
See below the WB image, lot number and protocol we used for liver using anti-GGH/Gamma Glutamyl Hydrolase antibody A03161. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-22
Question
Is a blocking peptide available for product anti-GGH/Gamma Glutamyl Hydrolase antibody (A03161)?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We do provide the blocking peptide for product anti-GGH/Gamma Glutamyl Hydrolase antibody (A03161). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-23
Question
I was wanting to use your anti-GGH/Gamma Glutamyl Hydrolase antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
As indicated on the product datasheet, A03161 anti-GGH/Gamma Glutamyl Hydrolase antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-28
Question
Will anti-GGH/Gamma Glutamyl Hydrolase antibody A03161 work on zebrafish WB with melanoma?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
Our lab technicians have not tested anti-GGH/Gamma Glutamyl Hydrolase antibody A03161 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-GGH/Gamma Glutamyl Hydrolase antibody A03161 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish melanoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-14
Question
We are currently using anti-GGH/Gamma Glutamyl Hydrolase antibody A03161 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
K. Rodriguez
Verified customer
Asked: 2017-05-29
Answer
The anti-GGH/Gamma Glutamyl Hydrolase antibody (A03161) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-29
Question
Will anti-GGH/Gamma Glutamyl Hydrolase antibody A03161 work for WB with liver?
E. Wu
Verified customer
Asked: 2017-02-16
Answer
According to the expression profile of liver, GGH is highly expressed in liver. So, it is likely that anti-GGH/Gamma Glutamyl Hydrolase antibody A03161 will work for WB with liver.
Boster Scientific Support
Answered: 2017-02-16
Question
you antibody to test anti-GGH/Gamma Glutamyl Hydrolase antibody A03161 on mouse liver for research purposes, then I may be interested in using anti-GGH/Gamma Glutamyl Hydrolase antibody A03161 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Bhatt
Verified customer
Asked: 2014-07-16
Answer
The products we sell, including anti-GGH/Gamma Glutamyl Hydrolase antibody A03161, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-07-16
